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Literature summary for 3.5.1.88 extracted from
- Structure of peptide deformylase and identification of the substrate binding site (1998), J. Biol. Chem., 273, 11413-11416.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| structure of the catalytically active enzyme in the nickel-bound form at 2.5-A resolution and at 1.9-A resolution in complex with the competitive inhibitor polyethylene glycol molecule | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Polyethylene glycol | - |
Escherichia coli |  |
| polylethylene glycol | competitive inhibitor with respect to formylmethionine | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.1 | - |
N-formyl-Met | - |
Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | enzyme contains Fe2+ | Escherichia coli | |
| Ni2+ | enzyme nearly retains its activity on substitution of the naturally occuring Fe2+ by Ni2+ | Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-formyl-L-methionine-polypeptide + H2O | Escherichia coli | the enzyme is requiredfor the removal of the formyl group at the N-terminus of nascent polypeptide chains | formate + L-methionine-polypeptide | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-formyl-L-methionine-polypeptide + H2O | the enzyme is requiredfor the removal of the formyl group at the N-terminus of nascent polypeptide chains | Escherichia coli | formate + L-methionine-polypeptide | - |
? | |
| N-formyl-Met + H2O | - |
Escherichia coli | formate + Met | - |
? | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 6 | - |
Polyethylene glycol | - |
Escherichia coli | |
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Release 2023.1 (January 2023)
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