Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.1.88 extracted from

  • Ragusa, S.; Mouchet, P.; Lazennec, C.; Dive, V.; Meinnel, T.
    Substrate recognition and selectivity of peptide deformylase. Similarities and differences with metzincins and thermolysin (1999), J. Mol. Biol., 289, 1445-1457.
    View publication on PubMed
Search for more literature for 3.5.1.88

Protein Variants

Protein Variants Comment Organism
C129A turnover-number is 86% of that of the wild-type enzyme, Km-value for N-formyl-Met-Ala is 1.3fold higher than the Km-value of the wild-type enzyme Escherichia coli
E88F turnover-number is 0.36% of that of the wild-type enzyme, Km-value for N-formyl-Met-Ala is 1.1fold higher than the Km-value of the wild-type enzyme Escherichia coli
I128A Km-value for N-formyl-Met-Ala is 8.4fold higher than the Km-value of the wild-type enzyme Escherichia coli
I128F turnover-number is 0.13% of that of the wild-type enzyme, Km-value for N-formyl-Met-Ala is 37% of the Km-value of the wild-type enzyme Escherichia coli
I128R turnover-number is 0.6% of that of the wild-type enzyme, Km-value for N-formyl-Met-Ala is 6fold higher than the Km-value of the wild-type enzyme Escherichia coli
I128S turnover-number is 11% of that of the wild-type enzyme, Km-value for N-formyl-Met-Ala is 1.25fold higher than the Km-value of the wild-type enzyme Escherichia coli
I130F turnover-number is 58% of that of the wild-type enzyme, Km-value for N-formyl-Met-Ala is 77% of the Km-value of the wild-type enzyme Escherichia coli
I44F turnover-number is 3.8% of that of the wild-type enzyme, Km-value for N-formyl-Met-Ala is 35% of the Km-value of the wild-type enzyme Escherichia coli
I86A turnover-number is 8.9% of that of the wild-type enzyme, Km-value for N-formyl-Met-Ala is 1.fold higher than the Km-value of the wild-type enzyme Escherichia coli
I86F turnover-number is 3.3% of that of the wild-type enzyme, Km-value for N-formyl-Met-Ala is 65% of the Km-value of the wild-type enzyme Escherichia coli
L125A turnover-number is 2.9% of that of the wild-type enzyme, Km-value for N-formyl-Met-Ala is 3fold higher than the Km-value of the wild-type enzyme Escherichia coli
L125W turnover-number is 0.9% of that of the wild-type enzyme, Km-value for N-formyl-Met-Ala is 1.9fold higher than the Km-value of the wild-type enzyme Escherichia coli
L126F turnover-number is 113% of that of the wild-type enzyme, Km-value for N-formyl-Met-Ala is 1.5fold higher than the Km-value of the wild-type enzyme Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
imino[(5-methoxy-5-oxo-4-[2-(sulfanylmethyl)hexanoyl]aminopentyl)amino]methaneamine
-
 Escherichia coli
methyl-1-(sulfanylmethyl)hexanoate
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.62
-
 N-formyl-Nle-Lys(NH2)
-
 Escherichia coli
0.66
-
 N-formyl-Nle-Arg(NH2)
-
 Escherichia coli
1
-
 N-formyl-Nle-Ser(NH2)
-
 Escherichia coli
1.3
-
 N-formyl-Nle-Ala(NH2)
-
 Escherichia coli
1.5
-
 N-formyl-Nle-Gln(NH2)
-
 Escherichia coli
1.6
-
 N-formyl-Nle-Thr(NH2)
-
 Escherichia coli
1.7
-
 N-formyl-Nle-Met(NH2)
-
 Escherichia coli
1.8
-
 N-formyl-Nle-Ser(NH2)
-
 Escherichia coli
1.8
-
 N-formyl-Nle-Leu(NH2)
-
 Escherichia coli
1.9
-
 N-formyl-Nle-Trp(NH2)
-
 Escherichia coli
1.9
-
 N-formyl-Nle-Val(NH2)
-
 Escherichia coli
2.9
-
 N-formyl-Nle-Nle(NH2)
-
 Escherichia coli
3
-
 N-formyl-Nle-Phe(NH2)
-
 Escherichia coli
3.3
-
 N-formyl-Nle-Tyr(NH2)
-
 Escherichia coli
3.7
-
 N-formyl-Nle-Pro(NH2)
-
 Escherichia coli
4.3
-
 N-formyl-Nle-Asp(NH2)
-
 Escherichia coli
4.3
-
 N-formyl-Nle-Glu(NH2)
-
 Escherichia coli
5.5
-
 N-formyl-Nle-Gly(NH2)
-
 Escherichia coli
8.2
-
 N-formyl-Nle-Asn(NH2)
-
 Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information overview Escherichia coli ?
-
 ?
N-formyl-Met-Ala + H2O
-
 Escherichia coli formate + Met-Ala
-
 ?
N-formyl-Nle-Ala(NH2) + H2O
-
 Escherichia coli formate + Nle-Ala(NH2)
-
 ?
N-formyl-Nle-Arg(NH2) + H2O
-
 Escherichia coli formate + Nle-Arg(NH2)
-
 ?
N-formyl-Nle-Asn(NH2) + H2O
-
 Escherichia coli formate + Nle-Asn(NH2)
-
 ?
N-formyl-Nle-Asp(NH2) + H2O
-
 Escherichia coli formate + Nle-Asp(NH2)
-
 ?
N-formyl-Nle-Gln(NH2) + H2O
-
 Escherichia coli formate + Nle-Gln(NH2)
-
 ?
N-formyl-Nle-Glu(NH2) + H2O
-
 Escherichia coli formate + Nle-Glu(NH2)
-
 ?
N-formyl-Nle-Gly(NH2) + H2O
-
 Escherichia coli formate + Nle-Gly(NH2)
-
 ?
N-formyl-Nle-His(NH2) + H2O
-
 Escherichia coli formate + Nle-His(NH2)
-
 ?
N-formyl-Nle-Leu(NH2) + H2O
-
 Escherichia coli formate + Nle-Leu(NH2)
-
 ?
N-formyl-Nle-Lys(NH2) + H2O
-
 Escherichia coli formate + Nle-Lys(NH2)
-
 ?
N-formyl-Nle-Met(NH2) + H2O
-
 Escherichia coli formate + Nle-Met(NH2)
-
 ?
N-formyl-Nle-Nle(NH2) + H2O
-
 Escherichia coli formate + Nle-Nle(NH2)
-
 ?
N-formyl-Nle-Phe(NH2) + H2O
-
 Escherichia coli formate + Nle-Phe(NH2)
-
 ?
N-formyl-Nle-Pro(NH2) + H2O
-
 Escherichia coli formate + Nle-Pro(NH2)
-
 ?
N-formyl-Nle-Ser(NH2) + H2O
-
 Escherichia coli formate + Nle-Ser(NH2)
-
 ?
N-formyl-Nle-Thr(NH2) + H2O
-
 Escherichia coli formate + Nle-Thr(NH2)
-
 ?
N-formyl-Nle-Trp(NH2) + H2O
-
 Escherichia coli formate + Nle-Trp(NH2)
-
 ?
N-formyl-Nle-Tyr(NH2) + H2O
-
 Escherichia coli formate + Nle-Tyr(NH2)
-
 ?
N-formyl-Nle-Val(NH2) + H2O
-
 Escherichia coli formate + Nle-Val(NH2)
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
120
-
 N-formyl-Nle-Asp(NH2)
-
 Escherichia coli
130
-
 N-formyl-Nle-Trp(NH2)
-
 Escherichia coli
140
-
 N-formyl-Nle-Tyr(NH2)
-
 Escherichia coli
150
-
 N-formyl-Nle-Leu(NH2)
-
 Escherichia coli
150
-
 N-formyl-Nle-Pro(NH2)
-
 Escherichia coli
160
-
 N-formyl-Nle-Val(NH2)
-
 Escherichia coli
170
-
 N-formyl-Nle-Ser(NH2)
-
 Escherichia coli
180
-
 N-formyl-Nle-Thr(NH2)
-
 Escherichia coli
190
-
 N-formyl-Nle-Met(NH2)
-
 Escherichia coli
200
-
 N-formyl-Nle-Glu(NH2)
-
 Escherichia coli
200
-
 N-formyl-Nle-Phe(NH2)
-
 Escherichia coli
210
-
 N-formyl-Nle-Nle(NH2)
-
 Escherichia coli
230
-
 N-formyl-Nle-Lys(NH2)
-
 Escherichia coli
250
-
 N-formyl-Nle-His(NH2)
-
 Escherichia coli
260
-
 N-formyl-Nle-Gly(NH2)
-
 Escherichia coli
270
-
 N-formyl-Nle-Ala(NH2)
-
 Escherichia coli
280
-
 N-formyl-Nle-Gln(NH2)
-
 Escherichia coli
300
-
 N-formyl-Nle-Arg(NH2)
-
 Escherichia coli
320
-
 N-formyl-Nle-Asn(NH2)
-
 Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.04
-
 methyl-1-(sulfanylmethyl)hexanoate mutant enzyme I44F Escherichia coli
0.052
-
 methyl-1-(sulfanylmethyl)hexanoate wild-type enzyme and mutant enzyme E88A Escherichia coli
0.08
-
 methyl-1-(sulfanylmethyl)hexanoate mutant enzyme C129W Escherichia coli
0.18
-
 methyl-1-(sulfanylmethyl)hexanoate mutant enzyme L125W Escherichia coli
0.6
-
 methyl-1-(sulfanylmethyl)hexanoate mutant enzyme I86F Escherichia coli
0.64
-
 methyl-1-(sulfanylmethyl)hexanoate mutant enzyme L125A Escherichia coli
1.5
-
 methyl-1-(sulfanylmethyl)hexanoate mutant enzyme I1289S Escherichia coli
2
-
 methyl-1-(sulfanylmethyl)hexanoate mutant enzyme I128R Escherichia coli
10
-
 methyl-1-(sulfanylmethyl)hexanoate mutant enzyme I128F Escherichia coli