General Stability | Organism |
---|---|
denaturation with guanidinium hydrochloride, protein 1 unfolds at more than 3 M, protein 2 unfolds at 1.7 M | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | enzyme contains iron | Haemophilus influenzae | |
Fe | enzyme form 2 contains up to 1.0 iron atom per polypeptide - 0.77 Fe2+ and 0.2 Fe3+ per polypeptide. The iron is likely coordinated by His132, His136 and Cys90 in the protein | Escherichia coli | |
Zn2+ | enzyme form 1 contains approximately 1.0 Zn2+ per polypeptide chain, the zinc-containing protein 1 is likely formed during iron depletion caused by overexpression | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
19000 | - |
1 * 19000, SDS-PAGE | Escherichia coli |
19200 | - |
1 * 19200, electrospray ionization mass spectrometry | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Haemophilus influenzae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-formyl-Met-Ala-Ser + H2O | - |
Escherichia coli | formate + Met-Ala-Ser | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 19000, SDS-PAGE | Escherichia coli |
monomer | 1 * 19200, electrospray ionization mass spectrometry | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | - |
t1/2 is 1 min, enzyme form 2 | Escherichia coli |