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Literature summary for 3.5.1.88 extracted from

  • Guo, X.C.; Rajagopalan, P.T.R.; Pei, D.
    A direct spectrophotometric assay for peptide deformylase (1999), Anal. Biochem., 273, 298-304.
    View publication on PubMed
Search for more literature for 3.5.1.88

Application

Application Comment Organism
analysis novel class of N-formylated peptides for routine kinetic characterization and for screening PDF inhibitors Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 N-formylmethionyl-Leu-p-nitroanilide
-
 Escherichia coli
0.037
-
 N-formyl-beta-thiaphenylalanyl-Ala-Leu-4-nitroanilide
-
 Escherichia coli
0.53
-
 N-formyl-thiaphenylalanyl-peptide
-
 Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
formyl-L-Met-Leu-p-nitroanilide + H2O
-
 Escherichia coli formate + Met-Leu-p-nitroanilide
-
 ?
N-formyl-beta-thiaphenylalanyl-Ala-Leu-4-nitroanilide + H2O
-
 Escherichia coli formate + beta-thiaphenylalanyl-Ala-Leu-4-nitroanilide
-
 ?
N-formyl-beta-thiaphenylalanyl-Lys-p-nitroanilide + H2O
-
 Escherichia coli formate + beta-thiaphenylalanyl-Lys-p-nitroanilide
-
 ?
N-formyl-beta-thiaphenylalanyl-peptide + H2O significantly poorer substrate than N-formyl-thiaphenylalanylleucyl-p-nitroanilide Escherichia coli formate + beta-thiaphenylalanyl-peptide
-
 ?
N-formyl-beta-thiaphenylalanylleucyl 4-nitroanilide + H2O excellent substrate Escherichia coli formate + beta-thiaphenylalanylleucyl 4-nitroanilide
-
 ?
N-formyl-Met-Leu-p-nitroanilide + H2O best substrate Escherichia coli formate + Met-Leu-p-nitroanilide
-
 ?
N-formyl-thiaphenylalanyl-peptide + H2O
-
 Escherichia coli formate + thiaphenylalanyl-peptide
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5
-
 N-formyl-thiaphenylalanyl-peptide
-
 Escherichia coli
26
-
 N-formyl-beta-thiaphenylalanyl-Ala-Leu-4-nitroanilide
-
 Escherichia coli
38
-
 N-formyl-Met-Leu-p-nitroanilide
-
 Escherichia coli