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Literature summary for 3.5.1.88 extracted from
- Enzymatic properties of Escherichia coli peptide deformylase (1995), J. Bacteriol., 177, 1883-1887.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| KCl | 2fold increase within 0-0.5 M, beyond 0.5 M smaller extend of increase | Escherichia coli |  |
General Stability
| General Stability | Organism |
|---|---|
| extremely labile | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | complete inhibition at 5 mM | Escherichia coli | |
| 8-hydroxyquinoline | 50% inhibition at 2 mM | Escherichia coli | |
| N-formyl-L-methionylalanine | inhibition above 4 mM | Escherichia coli | |
| ZnCl2 | - |
Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | cruical for activity | Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, chromatography techniques | Escherichia coli |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Escherichia coli | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.8 | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-formyl-Ala-Gly-Ser-Glu + H2O | poor activity, preference of N-terminal methionine | Escherichia coli | formate + Ala-Gly-Ser-Glu | - |
? | |
| N-formyl-L-Met-Val + H2O | - |
Escherichia coli | formate + L-Met-Val | - |
? | |
| N-formyl-Met + H2O | poor activity | Escherichia coli | formate + Met | - |
? | |
| N-formyl-Met-Ala + H2O | - |
Escherichia coli | formate + Met-Ala | - |
? | |
| N-formyl-Met-Ala-Ser + H2O | higher activity than compared to di- or tetrapeptides | Escherichia coli | formate + Met-Ala-Ser | - |
? | |
| N-formyl-Met-Ser-Asn-Glu + H2O | decreasing activity for tetrapeptides | Escherichia coli | formate + Met-Ser-Asn-Glu | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
inactivation above | Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 11.2 | constant activity within | Escherichia coli |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 35 | - |
ZnCl2 | - |
Escherichia coli | |
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Release 2023.1 (January 2023)
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