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Literature summary for 3.5.1.88 extracted from
- Inhibition of bacterial peptide deformylase by biaryl acid analogs (2000), Arch. Biochem. Biophys., 375, 355-358.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | C-terminally histidine-tagged, PDF-H6, is well expressed and has identical physical and catalytic properties as the wild-type enzyme, N-terminally histidine-tagged enzyme shows drastically reduced catalytic activity | Escherichia coli |
General Stability
| General Stability | Organism |
|---|---|
| enhanced in vitro stability by binding of Zn2+ | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-butyl-5,7-dimethyl-3-[2''-(1H-tetrazol-5-ylmethyl)-[1,1',4',1'']terphenyl-4-yl]-3H-imidazo[4,5-b]pyridine | belongs to a class of angiotensin II receptor antagonists, potent inhibitor, IC50: 0.0076 mM | Escherichia coli |  |
| 3-[5'-benzyl-2'-(1H-tetrazol-5-yl)-biphenyl-4-yl]-2-ethyl-5,7-dimethyl-3H-imidazo[4,5-b]pyridine | potent inhibitor, IC50: 0.0342 mM, the acidic moiety forms direct ionic interactions with active site metal cation | Escherichia coli | |
| 5-chloro-2-propyl-3-[2''-(1H-tetrazol-5-ylmethyl)-[1,1',4',1'']terphenyl-4-yl]-3H-imidazo[4,5-b]pyridine | belongs to a broad class of angiotensin II receptor antagonists, potent competitive inhibitor, IC50: 0.0039 mM, the acidic moiety forms direct ionic interactions with active site metal cation | Escherichia coli | |
| butyl {[4'-(2-ethyl-5,7-dimethyl-3H-imidazo[4,5-b]pyridin-3-yl)-5-(2-methylbutan-2-yl)biphenyl-2-yl]sulfonyl}carbamate | competitive inhibition, IC50: 0.015 mM, the acidic moiety forms direct ionic interactions with active site metal cation | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 8.8 | - |
N-formyl-L-Met-Val | - |
Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | 10fold more active than Co2+-prepared enzyme | Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-formyl-L-methionine-polypeptide + H2O | Escherichia coli | the enzyme is involved in maturation of proteins by cleaving the N-formyl group from N-blocked methionine polypeptides | formate + L-methionine-polypeptide | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
C-terminally truncated recombinant enzyme | - |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| sensitive to intracellular reactive oxygen | Escherichia coli |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, recombinant C-terminal truncated enzyme, preliminary Zn2+-bound, 10fold more active than Co2+-bound enzyme | Escherichia coli |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Escherichia coli | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-formyl-L-Met-Val + H2O | - |
Escherichia coli | formate + L-Met-Val | - |
? | |
| N-formyl-L-methionine-polypeptide + H2O | the enzyme is involved in maturation of proteins by cleaving the N-formyl group from N-blocked methionine polypeptides | Escherichia coli | formate + L-methionine-polypeptide | - |
? | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0012 | - |
3-[5'-benzyl-2'-(1H-tetrazol-5-yl)-biphenyl-4-yl]-2-ethyl-5,7-dimethyl-3H-imidazo[4,5-b]pyridine | - |
Escherichia coli | |
| 0.006 | - |
butyl {[4'-(2-ethyl-5,7-dimethyl-3H-imidazo[4,5-b]pyridin-3-yl)-5-(2-methylbutan-2-yl)biphenyl-2-yl]sulfonyl}carbamate | - |
Escherichia coli | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.0039 | - |
belongs to a broad class of angiotensin II receptor antagonists, potent competitive inhibitor, IC50: 0.0039 mM, the acidic moiety forms direct ionic interactions with active site metal cation | Escherichia coli | 5-chloro-2-propyl-3-[2''-(1H-tetrazol-5-ylmethyl)-[1,1',4',1'']terphenyl-4-yl]-3H-imidazo[4,5-b]pyridine | |
| 0.0076 | - |
belongs to a class of angiotensin II receptor antagonists, potent inhibitor, IC50: 0.0076 mM | Escherichia coli | 2-butyl-5,7-dimethyl-3-[2''-(1H-tetrazol-5-ylmethyl)-[1,1',4',1'']terphenyl-4-yl]-3H-imidazo[4,5-b]pyridine | |
| 0.015 | - |
competitive inhibition, IC50: 0.015 mM, the acidic moiety forms direct ionic interactions with active site metal cation | Escherichia coli | butyl {[4'-(2-ethyl-5,7-dimethyl-3H-imidazo[4,5-b]pyridin-3-yl)-5-(2-methylbutan-2-yl)biphenyl-2-yl]sulfonyl}carbamate | |
| 0.0342 | - |
potent inhibitor, IC50: 0.0342 mM, the acidic moiety forms direct ionic interactions with active site metal cation | Escherichia coli | 3-[5'-benzyl-2'-(1H-tetrazol-5-yl)-biphenyl-4-yl]-2-ethyl-5,7-dimethyl-3H-imidazo[4,5-b]pyridine | |
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