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Literature summary for 3.5.1.88 extracted from
- Peptide deformylase as a target for new generation, broad spectrum antimicrobial agents (2000), Mol. Microbiol., 36, 1197-1205.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | possible target for new broad spectrum antimicrobial agents | Staphylococcus aureus |
| medicine | possible target for new broad spectrum antimicrobial agents | Escherichia coli |
| medicine | possible target for new broad spectrum antimicrobial agents | Geobacillus stearothermophilus |
| medicine | possible target for new broad spectrum antimicrobial agents | Enterococcus faecalis |
| medicine | possible target for new broad spectrum antimicrobial agents | Plasmodium falciparum |
| medicine | possible target for new broad spectrum antimicrobial agents | Trypanosoma sp. |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of a C-terminal 21 amino acid truncated enzyme | Escherichia coli |
General Stability
| General Stability | Organism |
|---|---|
| enhanced in vitro stability by binding of Zn2+ | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| actinonin | - |
Arabidopsis thaliana |  |
| actinonin | pseudo-peptide hydroxamate, potent inhibitor Kd: 0.3 nM | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | sensitive to reactive oxygen | Escherichia coli | |
| Fe3+ | more stable activity than with Fe2+ | Escherichia coli | |
| Ni2+ | gives insensitivity to oxidation | Escherichia coli | |
| Zn2+ | cruical for activity | Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-formyl-L-methionine-polypeptide + H2O | Arabidopsis thaliana | - |
formate + L-methionine-polypeptide | - |
? | |
| N-formyl-L-methionine-polypeptide + H2O | Plasmodium falciparum | - |
formate + L-methionine-polypeptide | - |
? | |
| N-formyl-L-methionine-polypeptide + H2O | Trypanosoma sp. | - |
formate + L-methionine-polypeptide | - |
? | |
| N-formyl-L-methionine-polypeptide + H2O | Staphylococcus aureus | deformylation required for methionine aminopeptidase activity in eubacteria, highly specific | formate + L-methionine-polypeptide | - |
? | |
| N-formyl-L-methionine-polypeptide + H2O | Escherichia coli | deformylation required for methionine aminopeptidase activity in eubacteria, highly specific | formate + L-methionine-polypeptide | - |
? | |
| N-formyl-L-methionine-polypeptide + H2O | Geobacillus stearothermophilus | deformylation required for methionine aminopeptidase activity in eubacteria, highly specific | formate + L-methionine-polypeptide | - |
? | |
| N-formyl-L-methionine-polypeptide + H2O | Enterococcus faecalis | deformylation required for methionine aminopeptidase activity in eubacteria, highly specific | formate + L-methionine-polypeptide | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
- |
- |
| Enterococcus faecalis | - |
- |
- |
| Escherichia coli | - |
- |
- |
| Geobacillus stearothermophilus | - |
- |
- |
| no activity in Caenorhabditis elegans | - |
- |
- |
| no activity in Saccharomyces cerevisiae | - |
- |
- |
| Plasmodium falciparum | - |
- |
- |
| Solanum lycopersicum | - |
- |
- |
| Staphylococcus aureus | - |
- |
- |
| Trypanosoma sp. | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| sensitive to intracellular reactive oxygen | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-formyl-L-methionine-polypeptide + H2O | - |
Arabidopsis thaliana | formate + L-methionine-polypeptide | - |
? | |
| N-formyl-L-methionine-polypeptide + H2O | - |
Plasmodium falciparum | formate + L-methionine-polypeptide | - |
? | |
| N-formyl-L-methionine-polypeptide + H2O | - |
Trypanosoma sp. | formate + L-methionine-polypeptide | - |
? | |
| N-formyl-L-methionine-polypeptide + H2O | deformylation required for methionine aminopeptidase activity in eubacteria, highly specific | Staphylococcus aureus | formate + L-methionine-polypeptide | - |
? | |
| N-formyl-L-methionine-polypeptide + H2O | deformylation required for methionine aminopeptidase activity in eubacteria, highly specific | Escherichia coli | formate + L-methionine-polypeptide | - |
? | |
| N-formyl-L-methionine-polypeptide + H2O | deformylation required for methionine aminopeptidase activity in eubacteria, highly specific | Geobacillus stearothermophilus | formate + L-methionine-polypeptide | - |
? | |
| N-formyl-L-methionine-polypeptide + H2O | deformylation required for methionine aminopeptidase activity in eubacteria, highly specific | Enterococcus faecalis | formate + L-methionine-polypeptide | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Escherichia coli |
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