Literature summary for 3.5.1.59 extracted from

Luo, H.B.; Zheng, H.; Zimmerman, M.D.; Chruszcz, M.; Skarina, T.; Egorova, O.; Savchenko, A.; Edwards, A.M.; Minor, W.
Crystal structure and molecular modeling study of N-carbamoylsarcosine amidase Ta0454 from Thermoplasma acidophilum (2010), J. Struct. Biol., 169, 304-311.

Cloned(Commentary)

Cloned (Comment)	Organism
the small regulatory subunit is cloned, expressed and purified	Thermoplasma acidophilum

Crystallization (Commentary)

Crystallization (Comment)	Organism
structure solved and refined at a resolution of 2.35 A. The enzyme shares a similar fold and a highly conserved C-D-K-catalytic triad, Cys123, Asp9, and Lys90 with the structures of three cysteine hydrolases (PDB codes: 1NBA, 1IM5, and 2HOR). Molecular dynamics simulations of Ta0454/N-carbamoylsarcosine and Ta0454/pyrazinamide complexes are performed to determine the structural basis of the substrate binding pattern for each ligand. The predicted binding free energies suggest that Ta0454 is selective for N-carbamoylsarcosine over pyrazinamide, and zinc ions play an important role in the favorable substrate bound states	Thermoplasma acidophilum

Crystallization (Comment)

Organism

structure solved and refined at a resolution of 2.35 A. The enzyme shares a similar fold and a highly conserved C-D-K-catalytic triad, Cys123, Asp9, and Lys90 with the structures of three cysteine hydrolases (PDB codes: 1NBA, 1IM5, and 2HOR). Molecular dynamics simulations of Ta0454/N-carbamoylsarcosine and Ta0454/pyrazinamide complexes are performed to determine the structural basis of the substrate binding pattern for each ligand. The predicted binding free energies suggest that Ta0454 is selective for N-carbamoylsarcosine over pyrazinamide, and zinc ions play an important role in the favorable substrate bound states

Thermoplasma acidophilum

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zinc	zinc ions play an important role in the favorable substrate bound staes	Thermoplasma acidophilum

Organism

Organism	UniProt	Comment	Textmining
Thermoplasma acidophilum	Q9HKY9	-	-

Purification (Commentary)

Purification (Comment)	Organism
the small regulatory subunit is cloned, expressed and purified	Thermoplasma acidophilum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
N-carbamoylsarcosine + H2O	-	Thermoplasma acidophilum	sarcosine + CO2 + NH3	-	?

Synonyms

Synonyms	Comment	Organism
CSHase	-	Thermoplasma acidophilum
N-carbamoylsarcosine amidase Ta0454	-	Thermoplasma acidophilum

General Information

General Information	Comment	Organism
physiological function	involved in the degradation of creatinine	Thermoplasma acidophilum