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Literature summary for 3.5.1.59 extracted from
- Purification and characterization of a novel enzyme, N-carbamoylsarcosine amidohydrolase, from Pseudomonas putida 77 (1986), J. Biol. Chem., 261, 11832-11839.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Pseudomonas putida |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag2+ | - |
Pseudomonas putida |  |
| Cu2+ | - |
Pseudomonas putida | |
| Hg2+ | - |
Pseudomonas putida | |
| additional information | no inhibition by 2 mM MgCl2, FeSO4, CdCl2, MnCl2, BaCl2, FeCl3, CaCl2, PbCl2, DTT or 2-mercaptoethanol, 1 mM 1,10-phenanthroline, 2,2'-dipyridyl, hydrazine, phenylhydrazine, 8-hydroxyquinoline, 4 mM sarcosine, glycine, N-methyl-DL-valine, N-methyl-DL-alanine, N,N-dimethylglycine, N-methylleucine, methylurea, dimethylamine, trimethylamine or diethylamine | Pseudomonas putida | |
| NaN3 | weak | Pseudomonas putida | |
| PCMB | - |
Pseudomonas putida | |
| Zn2+ | weak | Pseudomonas putida | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.2 | - |
N-carbamoylsarcosine | - |
Pseudomonas putida | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27000 | - |
x * 27000, SDS-PAGE | Pseudomonas putida |
| 75000 | - |
HPLC gel filtration | Pseudomonas putida |
| 88000 | - |
gel filtration | Pseudomonas putida |
| 102000 | - |
sedimentation equilibrium method | Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-carbamoylsarcosine + H2O | Pseudomonas putida | creatine pathway | sarcosine + CO2 + NH3 | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| no glycoprotein | - |
Pseudomonas putida |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas putida |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.958 | - |
- |
Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | poor substrates are N-carbamoyl derivatives of DL-tryptophan, DL-phenylalanine, 4-hydroxy-DL-phenylglycine, D-valine, 4-methyl-DL-phenylglycine, DL-2-amino-n-butyric acid, DL-threonine or D-leucine. No substrates are N-carbamoyl derivatives of L-glutamic acid, L-valine, L-tyrosine, L-isoleucine, L-histidine, L-asparagine, DL-aspartic acid, L-serine, DL-methionine or 2-aminoisobutyric acid | Pseudomonas putida | ? | - |
? | |
| N-carbamoyl-D-alanine + H2O | hydrolysis at 7% the rate of N-carbamoylsarcosine | Pseudomonas putida | ? | - |
? | |
| N-carbamoylglycine + H2O | hydrolysis at 10% the rate of N-carbamoylsarcosine | Pseudomonas putida | ? | - |
? | |
| N-carbamoylsarcosine + H2O | best substrate | Pseudomonas putida | sarcosine + CO2 + NH3 | - |
ir | |
| N-carbamoylsarcosine + H2O | creatine pathway | Pseudomonas putida | sarcosine + CO2 + NH3 | - |
ir | |
| N-methyl-N-carbamoyl-D-alanine + H2O | hydrolysis at 13% the rate of N-carbamoylsarcosine | Pseudomonas putida | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 27000, SDS-PAGE | Pseudomonas putida |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Pseudomonas putida |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 50 | about half-maximal activity at 30°C and 50°C | Pseudomonas putida |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
10 min, stable at pH 6-7 | Pseudomonas putida |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | - |
Pseudomonas putida |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8.7 | about half-maximal activity at pH 5 and 8.7 | Pseudomonas putida |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7 | 10 min, stable at 40°C | Pseudomonas putida |
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