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Literature summary for 3.5.1.54 extracted from

  • Shapir, N.; Sadowsky, M.J.; Wackett, L.P.
    Purification and characterization of allophanate hydrolase (AtzF) from Pseudomonas sp. strain ADP (2005), J. Bacteriol., 187, 3731-3738.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.54

Cloned(Commentary)

Cloned (Comment) Organism
gene atzF, expression of the His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Pseudomonas sp.

Protein Variants

Protein Variants Comment Organism
K91A site-directed mutagenesis, inactive mutant Pseudomonas sp.
S165A site-directed mutagenesis, inactive mutant Pseudomonas sp.
S189A site-directed mutagenesis, inactive mutant Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
phenyl phosphorodiamidate
-
 Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5
-
 allophanate pH 9.5, recombinant enzyme Pseudomonas sp.
5.3
-
 malonamic acid pH 9.5, recombinant enzyme Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66223
-
 4 * 66223, sequence calculation Pseudomonas sp.
260000
-
 gel filtration, recombinant enzyme Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
allophanate + H2O Pseudomonas sp.
-
 NH3 + CO2
-
 ir

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. Q936X2 gene atzF
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
allophanate + H2O
-
 Pseudomonas sp. NH3 + CO2
-
 ?
allophanate + H2O
-
 Pseudomonas sp. NH3 + CO2
-
 ir
biuret + H2O
-
 Pseudomonas sp. ?
-
 ?
malonamic acid + H2O
-
 Pseudomonas sp. ?
-
 ?
malonamic acid + hydroxylamine hydroxylamine trapping activity Pseudomonas sp. malonohydroxamate + ?
-
 ?
additional information substrate specificity, no activity with methyl allophanate, hydantoic acid, oxamic acid, hydroxyurea, methyl carbamate, N-methylurea, acetylurea, 1-acetyl-2-thiourea, and semicarbazide, no activity with rhodanine, rhodanine-3-acetic acid, 3-aminorhodanine, (4R)-(-)-2-thioxo-4-thiazolidinecarboxylic acid, (-)-2-oxo-4-thiazolinecarboxylic acid, and 2-amino-5-bromothiazol Pseudomonas sp. ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 66223, sequence calculation Pseudomonas sp.

Synonyms

Synonyms Comment Organism
AtzF
-
 Pseudomonas sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
 malonamic acid pH 9.5, recombinant enzyme Pseudomonas sp.
16.4
-
 allophanate pH 9.5, recombinant enzyme Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
 recombinant enzyme Pseudomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
8 11.3 recombinant enzyme Pseudomonas sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2
-
 phenyl phosphorodiamidate pH 9.5, recombinant enzyme Pseudomonas sp.