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Literature summary for 3.5.1.5 extracted from
- Purification, characterization and immobilization of urease from Momordica charantia seeds (2011), Process Biochem., 46, 1486-1491.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 34 | - |
Urea | pH 7.0, temperature not specified in the publication | Momordica charantia |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 174500 | - |
native PAGE | Momordica charantia |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Methanol | 100% activity | Momordica charantia |
| phenol | 0.01% activity | Momordica charantia |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Momordica charantia | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| anion exchange chromatography, gel filtration | Momordica charantia |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | - |
Momordica charantia | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.02 | - |
4.04 micromol NH3/min/mg, pH 7.0, temperature not specified in the publication | Momordica charantia |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| urea + H2O | - |
Momordica charantia | 2 NH3 + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | conclusion from native PAGE | Momordica charantia |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| urease | - |
Momordica charantia |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 50 | broad temperatur optimum in the range of 35-50°C | Momordica charantia |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
retains more than 80% activity after incubation up to 60°C for 15 min | Momordica charantia |
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