Any feedback?
Literature summary for 3.5.1.5 extracted from
- Helicobacter pylori urease may exist in two forms: evidence from the kinetic studies (2009), J. Microbiol. Biotechnol., 19, 1565-1568.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| glycerol | optimum concentration: 15% | Helicobacter pylori |  |
| n-octylglucoside | optimum concentration: 0.1% | Helicobacter pylori | |
| PEG | optimum concentration: 0.5% | Helicobacter pylori | |
| SDS | optimum concentration: 0.02% | Helicobacter pylori | |
| Triton X-100 | optimum concentration: 0.05% | Helicobacter pylori | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | - |
Helicobacter pylori | |
| mercaptoethylamine | inactivates enzyme irreversibly | Helicobacter pylori | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | sigmoid curve at low urea concentration (below 0.1 mM). In the presence of glycerol or PEG (preservatives), the sigmoid pattern changes to a rectangular hyperbola, and urea hydrolysis is consistent with Michaelis-Menten kinetics. Based on the kinetics of urease in the presence of the preservatives, the two forms of urease i.e., (alpha beta)3 and (alpha beta)6 may well exist in Helicobacter pylori. The hyperbolic kinetics adopted by the dissociated form of urease may allow for maximal urea assimilation by Helicobacter pylori under acidic conditions and at low urea concentration | Helicobacter pylori | |
| 0.21 | - |
Urea | pH 6.8, 37°C, Vmax: 1200 micromol/min/mg, in the presence of preservatives PEG or glycerol | Helicobacter pylori | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29000 | - |
SDS-PAGE, subunits of the purified urease are shown to be stoichiometrically equal, with estimated molecular masses of 63 kDa and 29kDa | Helicobacter pylori |
| 63000 | - |
SDS-PAGE, subunits of the purified urease are shown to be stoichiometrically equal, with estimated molecular masses of 63 kDa and 29kDa | Helicobacter pylori |
| 580000 | - |
gel filtration | Helicobacter pylori |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Helicobacter pylori | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using NH4(SO4)2 precipitation, phenyl-Sepharose column chromatography and gel filtration | Helicobacter pylori |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| urea + H2O | - |
Helicobacter pylori | CO2 + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | purified enzyme may be a hexameric aggregate, consistent with the reported stoichiometry of (29-63 kDa) * 6 of Helicobacter pylori | Helicobacter pylori |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| urease | - |
Helicobacter pylori |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Helicobacter pylori |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
assay at | Helicobacter pylori |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
enzyme gets irreversibly inactivated at pH 4 | Helicobacter pylori |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
