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Literature summary for 3.5.1.5 extracted from

  • Kot, M.; Karcz, W.; Zaborska, W.
    5-Hydroxy-1,4-naphthoquinone (juglone) and 2-hydroxy-1,4-naphthoquinone (lawsone) influence on jack bean urease activity: Elucidation of the difference in inhibition activity (2010), Bioorg. Chem., 38, 132-137.
    View publication on PubMed
Search for more literature for 3.5.1.5

Inhibitors

Inhibitors Comment Organism Structure
1,4-Naphthoquinone
-
 Canavalia ensiformis
5-hydroxy-1,4-naphthoquinone 5-hydroxy-1,4-naphthoquinone, juglone, acts as a strong, time and concentration dependent inactivator of urease. The reactivation of juglone-modified urease shows the participation of reversible and irreversible contribution in the inactivation. In the presence of an excess of DTT, urease inactivated by juglone regains 70% of its activity. The reversible inactivation is attributed to oxidation of the essential urease thiols by reactive oxygen species (ROS) realizing during reduction of juglone to seminaphthoquinone. The irreversible contribution in the inhibition is assumed as an arylation of urease thiol groups by juglone Canavalia ensiformis

Organism

Organism UniProt Comment Textmining
Canavalia ensiformis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
urea + H2O
-
 Canavalia ensiformis CO2 + NH3
-
 ?

Synonyms

Synonyms Comment Organism
urease
-
 Canavalia ensiformis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
 assay at Canavalia ensiformis

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.025
-
 pH 7.7, temperature not provided Canavalia ensiformis 5-hydroxy-1,4-naphthoquinone
0.03
-
 pH 7.7, temperature not provided Canavalia ensiformis 1,4-Naphthoquinone