Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Acetohydroxamic acid | - |
Brucella suis bv. 1 | |
Hydroxyurea | - |
Brucella suis bv. 1 | |
Thiourea | - |
Brucella suis bv. 1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.6 | - |
Urea | by hyperbolic regression, Lineweaver-Burk: 5.24 mM | Brucella suis bv. 1 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
11129 | - |
x * 61054 + x * 11377 + x * 11129, calculated from sequence | Brucella suis bv. 1 |
11377 | - |
x * 61054 + x * 11377 + x * 11129, calculated from sequence | Brucella suis bv. 1 |
14000 | - |
x * 66000 + x * 15500 + x * 14000, SDS-PAGE | Brucella suis bv. 1 |
15500 | - |
x * 66000 + x * 15500 + x * 14000, SDS-PAGE | Brucella suis bv. 1 |
61054 | - |
x * 61054 + x * 11377 + x * 11129, calculated from sequence | Brucella suis bv. 1 |
66000 | - |
x * 66000 + x * 15500 + x * 14000, SDS-PAGE | Brucella suis bv. 1 |
130000 | 135000 | gel filtration | Brucella suis bv. 1 |
157000 | - |
nondenaturing PAGE | Brucella suis bv. 1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brucella suis bv. 1 | Q8FZW3 | urease subunit beta 2 | - |
Purification (Comment) | Organism |
---|---|
- |
Brucella suis bv. 1 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
540 | - |
- |
Brucella suis bv. 1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
urea + H2O | - |
Brucella suis bv. 1 | CO2 + 2 NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 61054 + x * 11377 + x * 11129, calculated from sequence | Brucella suis bv. 1 |
? | x * 66000 + x * 15500 + x * 14000, SDS-PAGE | Brucella suis bv. 1 |
Synonyms | Comment | Organism |
---|---|---|
urease | - |
Brucella suis bv. 1 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | 35 | - |
Brucella suis bv. 1 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 45 | - |
Brucella suis bv. 1 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
- |
Brucella suis bv. 1 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | - |
Brucella suis bv. 1 |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.77 | - |
Acetohydroxamic acid | competitive | Brucella suis bv. 1 | |
1.04 | - |
Hydroxyurea | competitive | Brucella suis bv. 1 | |
26.12 | - |
Thiourea | competitive | Brucella suis bv. 1 |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Brucella suis bv. 1 | - |
- |
5 |