Application | Comment | Organism |
---|---|---|
analysis | urea is estimated in different blood samples with the help of agar-immobilized urease and the results are consistent with those from clinical pathology laboratory through an autoanalyzer | Cajanus cajan |
General Stability | Organism |
---|---|
agar-immobilized enzyme showes good mechanical stability | Cajanus cajan |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.23 | - |
Urea | soluble enzyme | Cajanus cajan | |
5.07 | - |
Urea | agar-immobilized enzyme | Cajanus cajan |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cajanus cajan | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
urea + H2O | - |
Cajanus cajan | CO2 + NH3 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
soluble urease | Cajanus cajan |
60 | - |
agar-immobilized urease | Cajanus cajan |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 60 | 20°C: about 90% of maximal activity, 60°C: about 40% of maximal activity, soluble enzyme | Cajanus cajan |
40 | 70 | 40°C: about 50% of maximal activity, 70°C: about 80% of maximal activity, agar-immobilized enzyme | Cajanus cajan |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
pH 7.3, half-life of soluble enzyme is 21 days, half-life of agar-immobilized enzyme is 53 days | Cajanus cajan |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
soluble enzyme | Cajanus cajan |
7.5 | - |
agar-immobilized enzyme | Cajanus cajan |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8.5 | pH 6.0: about 75% of maximal activity, pH 8.5: about 80% of maximal activity, soluble enzyme | Cajanus cajan |
7 | 8 | pH 7.0: about 65% of maximal activity, pH 8.0: about 70% of maximal activity, agar-immobilized enzyme | Cajanus cajan |