Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Nickel | addition of Ni to purified apourease does not yield active enzyme, the apoenzyme is very slowly activated in vivo by addition of Ni2+ ions to Ni-free cell cultures, apourease activation is an energy-dependent process that is deactivated by cell disruption | Klebsiella aerogenes | |
Nickel | nickel metalloenzyme | Klebsiella aerogenes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
9000 | - |
alpha2beta4tau4, 2 * 72000 + 4 * 11000 + 4 * 9000, SDS-PAGE | Klebsiella aerogenes |
11000 | - |
alpha2beta4tau4, 2 * 72000 + 4 * 11000 + 4 * 9000, SDS-PAGE | Klebsiella aerogenes |
72000 | - |
alpha2beta4tau4, 2 * 72000 + 4 * 11000 + 4 * 9000, SDS-PAGE | Klebsiella aerogenes |
224000 | - |
gel filtration | Klebsiella aerogenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella aerogenes | - |
- |
- |
Purification (Comment) | Organism |
---|---|
apoenzyme | Klebsiella aerogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
urea + H2O | - |
Klebsiella aerogenes | CO2 + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
decamer | alpha2beta4tau4, 2 * 72000 + 4 * 11000 + 4 * 9000, SDS-PAGE | Klebsiella aerogenes |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
Ni substantially increase stability of the intact metalloprotein, Tm = 79°C compared with apoenzyme Tm = 62°C | Klebsiella aerogenes |