Cloned (Comment) | Organism |
---|---|
urease gene cluster | Mycobacterium tuberculosis |
General Stability | Organism |
---|---|
resistant to denaturation by 4 M urea | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Acetohydroxamate | - |
Mycobacterium tuberculosis | |
EDTA | no inhibition | Mycobacterium tuberculosis | |
Hydroxyurea | - |
Mycobacterium tuberculosis | |
NEM | - |
Mycobacterium tuberculosis | |
phenylphosphoramidate | - |
Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
Urea | - |
Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Nickel | contains about 6 Ni2+ ions per urease molecule | Mycobacterium tuberculosis | |
Nickel | nickel metalloenzyme | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
11000 | - |
x + 11000 + x * 11000 + x * 62000, SDS-PAGE | Mycobacterium tuberculosis |
62000 | - |
x + 11000 + x * 11000 + x * 62000, SDS-PAGE | Mycobacterium tuberculosis |
185000 | - |
gel filtration | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
urea + H2O | Mycobacterium tuberculosis | expression increases 10fold upon nitrogen deprivation, the enzyme is involved in nitrogen aquisition by the bacterium | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Mycobacterium tuberculosis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
101 | - |
- |
Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
urea + H2O | - |
Mycobacterium tuberculosis | CO2 + NH3 | - |
? | |
urea + H2O | expression increases 10fold upon nitrogen deprivation, the enzyme is involved in nitrogen aquisition by the bacterium | Mycobacterium tuberculosis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x + 11000 + x * 11000 + x * 62000, SDS-PAGE | Mycobacterium tuberculosis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
30 min, stable up to | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Mycobacterium tuberculosis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8 | pH 5.5: about 55% of maximal activity, pH 8.0: about 65% of maximal activity | Mycobacterium tuberculosis |