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Literature summary for 3.5.1.5 extracted from

  • Clemens, D.L.; Lee, B.Y.; Horwitz, M.A.
    Purification, characterization, and genetic analysis of Mycobacterium tuberculosis urease, a potentially critical determinant of host-pathogen interaction (1995), J. Bacteriol., 177, 5644-5652.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
urease gene cluster Mycobacterium tuberculosis

General Stability

General Stability Organism
resistant to denaturation by 4 M urea Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
Acetohydroxamate
-
Mycobacterium tuberculosis
EDTA no inhibition Mycobacterium tuberculosis
Hydroxyurea
-
Mycobacterium tuberculosis
NEM
-
Mycobacterium tuberculosis
phenylphosphoramidate
-
Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
Urea
-
Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Nickel contains about 6 Ni2+ ions per urease molecule Mycobacterium tuberculosis
Nickel nickel metalloenzyme Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
11000
-
x + 11000 + x * 11000 + x * 62000, SDS-PAGE Mycobacterium tuberculosis
62000
-
x + 11000 + x * 11000 + x * 62000, SDS-PAGE Mycobacterium tuberculosis
185000
-
gel filtration Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
urea + H2O Mycobacterium tuberculosis expression increases 10fold upon nitrogen deprivation, the enzyme is involved in nitrogen aquisition by the bacterium ?
-
?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
101
-
-
Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
urea + H2O
-
Mycobacterium tuberculosis CO2 + NH3
-
?
urea + H2O expression increases 10fold upon nitrogen deprivation, the enzyme is involved in nitrogen aquisition by the bacterium Mycobacterium tuberculosis ?
-
?

Subunits

Subunits Comment Organism
? x + 11000 + x * 11000 + x * 62000, SDS-PAGE Mycobacterium tuberculosis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
30 min, stable up to Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
Mycobacterium tuberculosis

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8 pH 5.5: about 55% of maximal activity, pH 8.0: about 65% of maximal activity Mycobacterium tuberculosis