Crystallization (Comment) | Organism |
---|---|
wild-type and mutant enzyme | Klebsiella aerogenes |
Protein Variants | Comment | Organism |
---|---|---|
D221A | low activity, small increase in Km-value and pH 5 optimum | Klebsiella aerogenes |
H219A | 1000fold increased Km-value over that of the native enzyme | Klebsiella aerogenes |
H219H | 100fold increased Km-value over that of the native enzyme | Klebsiella aerogenes |
H219Q | 100fold increased Km-value over that of the native enzyme | Klebsiella aerogenes |
H320A | 100000fold deficiencies in rates, modest Km changes, disorders in the peptide flap covering their active sites, the pH-optimum is anomalous with optima near pH 6 and shoulders that extend to pH 9 | Klebsiella aerogenes |
H320N | 100000fold deficiencies in rates, modest Km changes, disorders in the peptide flap covering their active sites, the pH-optimum is anomalous with optima near pH 6 and shoulders that extend to pH 9 | Klebsiella aerogenes |
H320Q | 100000fold deficiencies in rates, modest Km changes, disorders in the peptide flap covering their active sites, the pH-optimum is anomalous with optima near pH 6 and shoulders that extend to pH 9 | Klebsiella aerogenes |
R336Q | 0.0001fold decreased catalytic rate with near-normal pH dependence, unaffected Km-value, phenylglyoxal inactivates at over half the rate observed for the native enzyme | Klebsiella aerogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.4 | - |
Urea | wild-type enzyme | Klebsiella aerogenes | |
2.7 | - |
Urea | mutant R336Q | Klebsiella aerogenes | |
3.6 | - |
Urea | mutant D221N | Klebsiella aerogenes | |
7.4 | - |
Urea | mutant H320N | Klebsiella aerogenes | |
10.6 | - |
Urea | mutant H320Q | Klebsiella aerogenes | |
10.9 | - |
Urea | mutant H320A | Klebsiella aerogenes | |
24 | - |
Urea | mutant D221A | Klebsiella aerogenes | |
175 | - |
Urea | mutant H219N | Klebsiella aerogenes | |
227 | - |
Urea | mutant H219Q | Klebsiella aerogenes | |
2090 | - |
Urea | mutant H219A | Klebsiella aerogenes |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Nickel | dinuclear nickel active site | Klebsiella aerogenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella aerogenes | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
urea + H2O = CO2 + 2 NH3 | mechanism | Klebsiella aerogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
urea + H2O | - |
Klebsiella aerogenes | CO2 + NH3 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
Urea | mutant H320N | Klebsiella aerogenes | |
0.068 | - |
Urea | mutant H320A | Klebsiella aerogenes | |
0.33 | - |
Urea | mutant H320Q | Klebsiella aerogenes | |
0.62 | - |
Urea | mutant R336Q | Klebsiella aerogenes | |
1.7 | - |
Urea | mutant D221A | Klebsiella aerogenes | |
60 | - |
Urea | mutant D221N | Klebsiella aerogenes | |
194 | - |
Urea | mutant H219A | Klebsiella aerogenes | |
322 | - |
Urea | mutant H219N | Klebsiella aerogenes | |
1860 | - |
Urea | mutant H219Q | Klebsiella aerogenes | |
2970 | - |
Urea | wild-type enzyme | Klebsiella aerogenes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
mutant D221A | Klebsiella aerogenes |
6 | - |
mutant enzymes H320A, H320N and H320A | Klebsiella aerogenes |
8 | - |
wild-type enzyme | Klebsiella aerogenes |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | about 65% of maximal activity at pH 7.0 and 9.0 | Klebsiella aerogenes |