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Literature summary for 3.5.1.49 extracted from
- The AmiE aliphatic amidase and AmiF formamidase of Helicobacter pylori: natural evolution of two enzyme paralogues (2001), Mol. Microbiol., 40, 596-609.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Helicobacter pylori |
| expression in Escherichia coli | Helicobacter pylori |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C166A | no enzymic activity | Helicobacter pylori |
| C166S | no enzymic activity | Helicobacter pylori |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| iodoacetate | 2.5 mM, 55% inhibition | Helicobacter pylori |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 32 | - |
Formamide | pH 7.4, 30°C | Helicobacter pylori | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37300 | - |
4 * 3400, SDS-PAGE, 4 * 37300, deduced from gene sequence | Helicobacter pylori |
| 160000 | - |
gel filtration | Helicobacter pylori |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Helicobacter pylori | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein | Helicobacter pylori |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Helicobacter pylori | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1040 | - |
pH 7.4, 30°C | Helicobacter pylori |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| formamide + H2O | from AmiF gene, that exclusively hydrolyzes formamide, involved in nitrogen metabolism | Helicobacter pylori | formate + NH3 | - |
? | |
| formamide + H2O | unique and specific substrate | Helicobacter pylori | formate + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 3400, SDS-PAGE, 4 * 37300, deduced from gene sequence | Helicobacter pylori |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Helicobacter pylori |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Helicobacter pylori |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | - |
Helicobacter pylori |
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Release 2023.1 (January 2023)
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