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Literature summary for 3.5.1.49 extracted from
- Purification, properties and heterologous expression of formamidase from Methylophilus methylotrophus (1994), Microbiology, 140, 191-195.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Methylophilus methylotrophus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.1 | - |
Formamide | - |
Methylophilus methylotrophus |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Methylophilus methylotrophus | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 51000 | - |
alpha2, 2 * 51000, SDS-PAGE | Methylophilus methylotrophus |
| 123000 | - |
gel filtration | Methylophilus methylotrophus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methylophilus methylotrophus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity | Methylophilus methylotrophus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | induction of enzyme synthesis using formamide- or acetamide-containing media | Methylophilus methylotrophus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 37.4 | - |
- |
Methylophilus methylotrophus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetamide + H2O | very low activity | Methylophilus methylotrophus | acetic acid + NH3 | - |
? | |
| acrylamide + H2O | very low activity | Methylophilus methylotrophus | acrylic acid + NH3 | - |
? | |
| butyramide + H2O | very low activity | Methylophilus methylotrophus | butyric acid + NH3 | - |
? | |
| formamide + H2O | - |
Methylophilus methylotrophus | formate + NH3 | source of nitrogen for growth and contribution to energy economy of the cell | ? | |
| propionamide + H2O | very low activity | Methylophilus methylotrophus | propionic acid + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | alpha2, 2 * 51000, SDS-PAGE | Methylophilus methylotrophus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 60.7 | - |
Formamide | - |
Methylophilus methylotrophus | |
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