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Literature summary for 3.5.1.41 extracted from
- Isolation and characterization of a gene coding for chitin deacetylase specifically expressed during fruiting body development in the basidiomycete Flammulina velutipes and its expression in the yeast Pichia pastoris (2008), FEMS Microbiol. Lett., 289, 130-137.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into the yeast expression vector pPICZalphaA | Flammulina velutipes |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | increases reaction velocity to 138% | Flammulina velutipes |  |
| Co2+ | increases reaction velocity to 163% | Flammulina velutipes | |
| Cu2+ | inhibits reaction velocity significantly | Flammulina velutipes | |
| Ni2+ | inhibits reaction velocity significantly | Flammulina velutipes | |
| Zn2+ | increases reaction velocity to 129% | Flammulina velutipes | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
theoretical, ORF | Flammulina velutipes |
| 31000 | - |
recombinant protein, determined by SDS-PAGE | Flammulina velutipes |
| 31520 | - |
theoretical, recombinant protein | Flammulina velutipes |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Flammulina velutipes | Q1XGD4 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fruit body | - |
Flammulina velutipes | - |
| pileus | - |
Flammulina velutipes | - |
| root | - |
Flammulina velutipes | - |
| stipe | - |
Flammulina velutipes | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the recombinant FV-PDA catalyses deacetylation of N-acetyl-chitooligomers, from dimer to pentamer, glycol chitin and colloidal chitin | Flammulina velutipes | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| chitin deacetylase | - |
Flammulina velutipes |
| FV-PDA | - |
Flammulina velutipes |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
activity assay | Flammulina velutipes |
| 60 | - |
recombinant protein | Flammulina velutipes |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 80 | relative activity 40-20%, 60°C 100% | Flammulina velutipes |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
activity assay | Flammulina velutipes |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 12 | relative activity 50-90% | Flammulina velutipes |
| 6 | 7 | relative activity 100% | Flammulina velutipes |
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