Literature summary for 3.5.1.41 extracted from

Gauthier, C.; Clerisse, F.; Dommes, J.; Jaspar-Versali, M.F.
Characterization and cloning of chitin deacetylases from Rhizopus circinans (2008), Protein Expr. Purif., 59, 127-137.

Cloned(Commentary)

Cloned (Comment)	Organism
three chitin deacetylase cDNAs (RC, D2, and I3/2) are isolated from the cDNA library and sequenced. These cDNAs exhibit features characteristic of chitin deacetylase sequences: the presence of a polysaccharide deacetylase domain, a metal-binding triad, the conserved catalytic residues, and high homology with various chitin deacetylase genes. The cDNAs are cloned in a Pichia pastoris expression system and produced as polyhistidine-tagged proteins. Only recombinant enzyme chitin deacetylase RC is active under the tested conditions	Rhizopus circinans

Cloned (Comment)

Organism

three chitin deacetylase cDNAs (RC, D2, and I3/2) are isolated from the cDNA library and sequenced. These cDNAs exhibit features characteristic of chitin deacetylase sequences: the presence of a polysaccharide deacetylase domain, a metal-binding triad, the conserved catalytic residues, and high homology with various chitin deacetylase genes. The cDNAs are cloned in a Pichia pastoris expression system and produced as polyhistidine-tagged proteins. Only recombinant enzyme chitin deacetylase RC is active under the tested conditions

Rhizopus circinans

General Stability

General Stability	Organism
good stability in the presence of acetate. Addition of 10 mM or 50 mM acetate to the assay mixture results, respectively, in a 4% or 49% loss of enzyme activity	Rhizopus circinans

Inhibitors

Inhibitors	Comment	Organism	Structure
Cu2+	10 mM, 41% inhibition	Rhizopus circinans

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	1 mM, slightly enhances activity	Rhizopus circinans
Mn2+	1 mM, slightly enhances activity	Rhizopus circinans

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
75000	-	x * 75000, SDS-PAGE	Rhizopus circinans

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
chitin + H2O	Rhizopus circinans	catalyzes hydrolysis of the acetamido groups of N-acetylglucosamine of chitin in fungal cell walls	chitosan + acetate	-	?

Organism

Organism	UniProt	Comment	Textmining
Rhizopus circinans	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	N-deglycosylation results in total loss of the enzyme activity	Rhizopus circinans

Purification (Commentary)

Purification (Comment)	Organism
-	Rhizopus circinans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
965.1	-	-	Rhizopus circinans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
chitin + H2O	catalyzes hydrolysis of the acetamido groups of N-acetylglucosamine of chitin in fungal cell walls	Rhizopus circinans	chitosan + acetate	-	?
chitin + H2O	enzyme exhibits high activity on fungal chitosan (78%), shrimp chitosan (83%), partially deacetylated chitin (77%), and chitohexaose (120%)	Rhizopus circinans	?	-	?
glycol chitin + H2O	-	Rhizopus circinans	chitosan + acetate	-	?

Subunits

Subunits	Comment	Organism
?	x * 75000, SDS-PAGE	Rhizopus circinans

Synonyms

Synonyms	Comment	Organism
chitin deacetylase RC	-	Rhizopus circinans

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	-	Rhizopus circinans

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	-	Rhizopus circinans