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Literature summary for 3.5.1.41 extracted from
- Purification of heat-stable deacetylase from Aspergillus nidulans and its role in cell wall degradation (1995), Curr. Microbiol., 30, 49-54.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| acetate | 0.4-4 mM, 4fold increase in activity | Aspergillus nidulans |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 40 mM, 100% inhibition | Aspergillus nidulans | |
| Ca2+ | 40 mM, 53% inhibition | Aspergillus nidulans | |
| Cd2+ | 40 mM, 50% inhibition | Aspergillus nidulans | |
| citrate | 40 mM, 44% inhibition | Aspergillus nidulans | |
| CN- | 40 mM, 56% inhibition | Aspergillus nidulans | |
| Co2+ | 40 mM, 42% inhibition | Aspergillus nidulans | |
| EDTA | 40 mM, 56% inhibition | Aspergillus nidulans | |
| Mg2+ | 40 mM, 39% inhibition | Aspergillus nidulans | |
| Mn2+ | 40 mM, 56% inhibition | Aspergillus nidulans | |
| NH4+ | 40 mM, 30% inhibition | Aspergillus nidulans | |
| Pb2+ | 40 mM, 67% inhibition | Aspergillus nidulans | |
| Sn2+ | 40 mM, 52% inhibition | Aspergillus nidulans | |
| Tartrate | 40 mM, 22% inhibition | Aspergillus nidulans | |
| Zn2+ | 40 mM, 64% inhibition | Aspergillus nidulans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.09 | - |
GlcNAc-beta-(1-4)-GlcNAc | 37°C | Aspergillus nidulans | |
| 0.12 | - |
GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc | 37°C | Aspergillus nidulans | |
| 0.15 | - |
GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc | 37°C | Aspergillus nidulans | |
| 0.5 | - |
GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc | 37°C | Aspergillus nidulans | |
| 2.5 | - |
GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc | 37°C | Aspergillus nidulans | |
| 2.96 | - |
glycol-chitin | 37°C | Aspergillus nidulans | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Aspergillus nidulans | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27300 | - |
gel filtration | Aspergillus nidulans |
| 27500 | - |
x * 27500, SDS-PAGE | Aspergillus nidulans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus nidulans | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | contains 28% (w/w) carbohydrate | Aspergillus nidulans |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus nidulans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Aspergillus nidulans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GlcNAc-beta-(1-4)-GlcNAc + H2O | 20% of the Vmax obtained with glycol-chitin | Aspergillus nidulans | acetate + ? | - |
? | |
| GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc + H2O | 27% of the Vmax obtained with glycol-chitin | Aspergillus nidulans | acetate + ? | - |
? | |
| GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc + H2O | 50% of the Vmax obtained with glycol-chitin | Aspergillus nidulans | acetate + ? | - |
? | |
| GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc + H2O | Vmax is 1.67fold higher compated to glycol-chitin | Aspergillus nidulans | acetate + ? | - |
? | |
| GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc + H2O | 30% of the Vmax obtained with glycol-chitin | Aspergillus nidulans | acetate + ? | - |
? | |
| glycol-chitin + H2O | - |
Aspergillus nidulans | ? + acetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 27500, SDS-PAGE | Aspergillus nidulans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Aspergillus nidulans |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 80 | 50°C: maximum, 80°C: 15% of maximal activity | Aspergillus nidulans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 100 | 1 h, stable | Aspergillus nidulans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Aspergillus nidulans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 7.5 | - |
Aspergillus nidulans |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Aspergillus nidulans | - |
- |
2.75 |
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