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Literature summary for 3.5.1.41 extracted from
- Purification and characterization of extracellular chitin deacetylase from Colletotrichum lindemuthianum (1996), Biosci. Biotechnol. Biochem., 60, 1598-1603.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Colletotrichum lindemuthianum |  |
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | industrial production of chitosan | Colletotrichum lindemuthianum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | at 10 mM | Colletotrichum lindemuthianum | |
| Cu2+ | - |
Colletotrichum lindemuthianum | |
| Fe2+ | - |
Colletotrichum lindemuthianum | |
| Mn2+ | - |
Colletotrichum lindemuthianum | |
| Ni2+ | - |
Colletotrichum lindemuthianum | |
| Zn2+ | - |
Colletotrichum lindemuthianum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.414 | - |
(N-acetyl-D-glucosamine)5 | - |
Colletotrichum lindemuthianum | |
| 0.6 | - |
(N-acetyl-D-glucosamine)4 | - |
Colletotrichum lindemuthianum | |
| 2.55 | - |
glycol chitin | - |
Colletotrichum lindemuthianum | |
| 11 | - |
(N-acetyl-D-glucosamine)3 | - |
Colletotrichum lindemuthianum | |
| 18.4 | - |
(N-acetyl-D-glucosamine)2 | - |
Colletotrichum lindemuthianum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Colletotrichum lindemuthianum | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | at 1 mM | Colletotrichum lindemuthianum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31500 | - |
SDS-PAGE | Colletotrichum lindemuthianum |
| 33000 | - |
gel filtration | Colletotrichum lindemuthianum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chitin + H2O | Colletotrichum lindemuthianum | - |
chitosan + acetate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Colletotrichum lindemuthianum | - |
ATCC 56676 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Colletotrichum lindemuthianum |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 944fold to homogeneity | Colletotrichum lindemuthianum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture filtrate | - |
Colletotrichum lindemuthianum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 18.4 | - |
- |
Colletotrichum lindemuthianum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C | Colletotrichum lindemuthianum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (N-acetyl-D-glucosamine)2 + H2O | - |
Colletotrichum lindemuthianum | (GlcN)GlcNAc + acetate | - |
? | |
| (N-acetyl-D-glucosamine)3 | - |
Colletotrichum lindemuthianum | (GlcN)2GlcNAc + acetate | - |
? | |
| (N-acetyl-D-glucosamine)4 | - |
Colletotrichum lindemuthianum | (GlcN)3GlcNAc + acetate | - |
? | |
| (N-acetyl-D-glucosamine)4 + H2O | - |
Colletotrichum lindemuthianum | (GlcN)3GlcNAc + acetate | - |
? | |
| (N-acetyl-D-glucosamine)5 | - |
Colletotrichum lindemuthianum | (GlcN)4GlcNAc + acetate | - |
? | |
| (N-acetyl-D-glucosamine)5 + H2O | - |
Colletotrichum lindemuthianum | (GlcN)4GlcNAc + acetate | - |
? | |
| chitin + H2O | - |
Colletotrichum lindemuthianum | chitosan + acetate | - |
? | |
| chitin + H2O | partially N-deacetylated water soluble chitin | Colletotrichum lindemuthianum | chitosan + acetate | - |
? | |
| glycol chitin + H2O | - |
Colletotrichum lindemuthianum | chitosan + acetate | - |
? | |
| N,N',N'',N''',N''''-pentaacetylchitopentaose + 5 H2O | - |
Colletotrichum lindemuthianum | chitopentaose + 5 acetate | end product | ? | |
| N,N',N'',N'''-tetraacetylchitotetraose + H2O | - |
Colletotrichum lindemuthianum | ? | - |
? | |
| N,N',N''-triacetylchitotriose + H2O | - |
Colletotrichum lindemuthianum | ? | - |
? | |
| N,N'-diacetylchitobiose + H2O | - |
Colletotrichum lindemuthianum | deacetylated chitooligosaccharides + acetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 31500-33000, SDS-PAGE | Colletotrichum lindemuthianum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Colletotrichum lindemuthianum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Colletotrichum lindemuthianum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Colletotrichum lindemuthianum |
| 45 | - |
stable | Colletotrichum lindemuthianum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.94 | - |
(N-acetyl-D-glucosamine)3 | - |
Colletotrichum lindemuthianum | |
| 8.52 | - |
(N-acetyl-D-glucosamine)2 | - |
Colletotrichum lindemuthianum | |
| 27.2 | - |
glycol chitin | - |
Colletotrichum lindemuthianum | |
| 83.8 | - |
(N-acetyl-D-glucosamine)5 | - |
Colletotrichum lindemuthianum | |
| 96.9 | - |
(N-acetyl-D-glucosamine)4 | - |
Colletotrichum lindemuthianum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 11.5 | 12 | - |
Colletotrichum lindemuthianum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Colletotrichum lindemuthianum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Colletotrichum lindemuthianum |
| 5 | 10.5 | - |
Colletotrichum lindemuthianum |
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