Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-diazo-4-oxo-D-norvaline | 40% inhibition at 2 mM | Achromobacter sp. | |
5-Diazo-4-oxo-L-norvaline | 50% inhibition at 0.6 mM | Achromobacter sp. | |
6-diazo-5-oxo-L-norleucine | irreversible inhibition at very low concentrations | Achromobacter sp. | |
Bromocresol green | 52-66% inhibition at 1 mM | Achromobacter sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
L-glutamine | - |
Achromobacter sp. | |
0.005 | - |
L-asparagine | - |
Achromobacter sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-asparagine + H2O | Achromobacter sp. | - |
L-aspartate + NH3 | - |
? | |
L-glutamine + H2O | Achromobacter sp. | higher activity for glutaminase than for asparaginase | L-glutamate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Achromobacter sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneous crystals | Achromobacter sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | highest enzyme formation in L-glutamic acid containing medium | Achromobacter sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
190 | - |
- |
Achromobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-cyanoalanine + H2O | slow hydrolysis rate | Achromobacter sp. | alanine + NH3 | - |
? | |
D-asparagine + H2O | one third of the L-isomer | Achromobacter sp. | D-aspartate + NH3 | - |
? | |
D-glutamine + H2O | one third of the L-isomer | Achromobacter sp. | D-glutamate + NH3 | - |
? | |
gamma-L-glutamyl hydroxylammonium sulfate + H2O | - |
Achromobacter sp. | L-glutamate + hydroxylammonium sulfate | - |
? | |
L-asparagine + H2O | - |
Achromobacter sp. | L-aspartate + NH3 | - |
? | |
L-glutamine + H2O | higher activity for glutaminase than for asparaginase | Achromobacter sp. | L-glutamate + NH3 | - |
? |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | - |
Achromobacter sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
5-Diazo-4-oxo-L-norvaline | - |
Achromobacter sp. |