Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.1.38 extracted from

  • Roberts, J.; Holcenberg, J.S.; Dolowy, W.C.
    Isolation, crystallization, and properties of Achromobacteraceae glutaminase-asparaginase with antitumor activity (1972), J. Biol. Chem., 247, 84-90.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
5-diazo-4-oxo-D-norvaline 40% inhibition at 2 mM Achromobacter sp.
5-Diazo-4-oxo-L-norvaline 50% inhibition at 0.6 mM Achromobacter sp.
6-diazo-5-oxo-L-norleucine irreversible inhibition at very low concentrations Achromobacter sp.
Bromocresol green 52-66% inhibition at 1 mM Achromobacter sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
L-glutamine
-
Achromobacter sp.
0.005
-
L-asparagine
-
Achromobacter sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-asparagine + H2O Achromobacter sp.
-
L-aspartate + NH3
-
?
L-glutamine + H2O Achromobacter sp. higher activity for glutaminase than for asparaginase L-glutamate + NH3
-
?

Organism

Organism UniProt Comment Textmining
Achromobacter sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneous crystals Achromobacter sp.

Source Tissue

Source Tissue Comment Organism Textmining
cell culture highest enzyme formation in L-glutamic acid containing medium Achromobacter sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
190
-
-
Achromobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-cyanoalanine + H2O slow hydrolysis rate Achromobacter sp. alanine + NH3
-
?
D-asparagine + H2O one third of the L-isomer Achromobacter sp. D-aspartate + NH3
-
?
D-glutamine + H2O one third of the L-isomer Achromobacter sp. D-glutamate + NH3
-
?
gamma-L-glutamyl hydroxylammonium sulfate + H2O
-
Achromobacter sp. L-glutamate + hydroxylammonium sulfate
-
?
L-asparagine + H2O
-
Achromobacter sp. L-aspartate + NH3
-
?
L-glutamine + H2O higher activity for glutaminase than for asparaginase Achromobacter sp. L-glutamate + NH3
-
?

pH Range

pH Minimum pH Maximum Comment Organism
6 9
-
Achromobacter sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.6
-
5-Diazo-4-oxo-L-norvaline
-
Achromobacter sp.