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Literature summary for 3.5.1.38 extracted from
- Extracellular asparaginase-glutaminase of Tilachlidium humicola (1973), J. Takeda Res. Lab., 32, 140-151.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | slight inhibition at 1 mM | Tilachlidium humicola |  |
| D-asparagine | mixed type of competitive and non-competitive inhibition, Ki: 7.2-8.4 mM | Tilachlidium humicola | |
| EDTA | slight inhibition at 8 mM | Tilachlidium humicola | |
| Hg2+ | slight inhibition at 1 mM | Tilachlidium humicola | |
| KCN | slight inhibition at 8 mM | Tilachlidium humicola | |
| NaAsO2 | 90% inhibition at 8 mM | Tilachlidium humicola | |
| NaF | slight inhibition at 8 mM | Tilachlidium humicola | |
| Ni2+ | 33% inhibition at 1 mM | Tilachlidium humicola | |
| p-chloromercuribenzoate | slight inhibition | Tilachlidium humicola | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.3 | - |
L-asparagine | - |
Tilachlidium humicola | |
| 0.6 | - |
L-glutamine | - |
Tilachlidium humicola | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Tilachlidium humicola | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe3+ | 30% activation at 1 mM | Tilachlidium humicola | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-asparagine + H2O | Tilachlidium humicola | - |
L-aspartate + NH3 | - |
? | |
| L-glutamine + H2O | Tilachlidium humicola | lower activity than for L-asparagine | L-glutamate + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Tilachlidium humicola | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Tilachlidium humicola |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | enzyme formation affected by culture conditions | Tilachlidium humicola | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 17.2 | - |
- |
Tilachlidium humicola |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-asparagine + H2O | - |
Tilachlidium humicola | D-aspartate + NH3 | - |
? | |
| D-glutamine + H2O | low activity | Tilachlidium humicola | D-glutamate + NH3 | - |
? | |
| L-asparagine + H2O | - |
Tilachlidium humicola | L-aspartate + NH3 | - |
? | |
| L-glutamine + H2O | lower activity than for L-asparagine | Tilachlidium humicola | L-glutamate + NH3 | - |
? | |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 55 | - |
Tilachlidium humicola |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
stable within 16 h between pH 4-10 | Tilachlidium humicola |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
less stable than at pH 5.6 or pH 10 | Tilachlidium humicola |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7.2 | 8.4 | D-asparagine | mixed type of competitive and non-competitive inhibition | Tilachlidium humicola | |
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