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Literature summary for 3.5.1.38 extracted from
- L-Asparaginases from Citrobacter freundii (1977), Biochim. Biophys. Acta, 480, 282-294.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| p-chloromercuribenzoate | complete inhibition at 0.1 mM, asparaginases A and B | Citrobacter freundii |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.029 | - |
L-asparagine | asparaginase A | Citrobacter freundii | |
| 1.7 | - |
L-asparagine | asparaginase B | Citrobacter freundii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
4 * 33000, SDS-PAGE | Citrobacter freundii |
| 140000 | - |
asparaginase A, gel filtration | Citrobacter freundii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-asparagine + H2O | Citrobacter freundii | - |
L-aspartate + NH3 | - |
? | |
| L-glutamine + H2O | Citrobacter freundii | low activity | L-glutamate + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Citrobacter freundii | - |
3 forms: asparaginase-glutaminase, asparaginase A, asparaginase B | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, asparaginase A | Citrobacter freundii |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Citrobacter freundii | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.6 | - |
asparaginase B | Citrobacter freundii |
| 133 | - |
asparaginase A | Citrobacter freundii |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 12 months, asparaginase A, asparaginase B: DTT required, 6 months | Citrobacter freundii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| aspartic acid + H2O | very low activity only for asparaginase B | Citrobacter freundii | ? | - |
? | |
| beta-D-aspartyl hydroxylammonium sulfate + H2O | only asparaginase A, low activity | Citrobacter freundii | L-Asp + hydroxylammonium sulfate | - |
? | |
| beta-L-aspartyl hydroxylammonium sulfate + H2O | higher activity for asparaginase B | Citrobacter freundii | L-Asp + hydroxylammonium sulfate | - |
? | |
| D-asparagine + H2O | low activity | Citrobacter freundii | D-aspartate + NH3 | - |
? | |
| D-glutamine + H2O | low activity | Citrobacter freundii | D-glutamate + NH3 | - |
? | |
| gamma-L-glutamyl hydroxylammonium sulfate + H2O | very low activities for both asparaginases A and B | Citrobacter freundii | L-glutamate + hydroxylammonium sulfate | - |
? | |
| L-asparagine + H2O | - |
Citrobacter freundii | L-aspartate + NH3 | - |
? | |
| L-glutamine + H2O | low activity | Citrobacter freundii | L-glutamate + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 33000, SDS-PAGE | Citrobacter freundii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| asparaginase A | - |
Citrobacter freundii |
| asparaginase B | - |
Citrobacter freundii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 51 | - |
asparaginase B, half-life: 10 min | Citrobacter freundii |
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