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Literature summary for 3.5.1.38 extracted from

  • Steckel, J.
    Kinetic properties and inhibition of Acinetobacter glutaminase-asparaginase (1983), Biochem. Pharmacol., 32, 971-977.
    View publication on PubMed
Search for more literature for 3.5.1.38

Inhibitors

Inhibitors Comment Organism Structure
6-diazo-5-oxo-L-norleucine competitive not reversible inhibition of both activities after extensive dialysis Acinetobacter glutaminasificans
acivicin competitive inhibition Acinetobacter glutaminasificans
aspartic acid competitive inhibition, Ki: glutaminase activity 0.16 mM, asparaginase activity 0.53 mM Acinetobacter glutaminasificans
azaserine competitive not reversible inhibition of both activities Acinetobacter glutaminasificans
glutamic acid competitive inhibition, Ki: glutaminase acitvity 5 mM, asparaginase activity 3 mM Acinetobacter glutaminasificans
L-asparagine competitive inhibition of glutaminase activity, Ki: 0.02 mM Acinetobacter glutaminasificans
L-methionine sulfoximine competitive inhibition Acinetobacter glutaminasificans
NH4Cl weak non-competitive inhibition of glutaminase activity Acinetobacter glutaminasificans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.041
-
 L-glutamine
-
 Acinetobacter glutaminasificans
0.055
-
 L-asparagine
-
 Acinetobacter glutaminasificans
0.083
-
 L-glutamine for succinylated enzyme Acinetobacter glutaminasificans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-asparagine + H2O Acinetobacter glutaminasificans
-
 L-aspartate + NH3
-
 ?
L-glutamine + H2O Acinetobacter glutaminasificans
-
 L-glutamate + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Acinetobacter glutaminasificans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity Acinetobacter glutaminasificans

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Acinetobacter glutaminasificans
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
150
-
-
 Acinetobacter glutaminasificans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-asparagine + H2O
-
 Acinetobacter glutaminasificans L-aspartate + NH3
-
 ?
L-glutamine + H2O
-
 Acinetobacter glutaminasificans L-glutamate + NH3
-
 ?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.02
-
 L-asparagine competitive inhibition of glutaminase activity Acinetobacter glutaminasificans