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Literature summary for 3.5.1.38 extracted from

  • Ortlund, E.; Lacount, M.W.; Lewinski, K.; Lebioda, L.
    Reactions of Pseudomonas 7A glutaminase-asparaginase with diazo analogues of glutamine and asparagine result in unexpected covalent inhibitions and suggests an unusual catalytic triad Thr-Tyr-Glu (2000), Biochemistry, 39, 1199-1204.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, in presence of inhibitors Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
5-Diazo-4-oxo-L-norvaline inactivation by covalent binding to Thr20 and Tyr34 Pseudomonas sp.
6-diazo-5-oxo-L-norleucine inactivation by covalent binding to Thr20 and Tyr34 Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-asparagine + H2O Pseudomonas sp.
-
L-aspartate + NH3
-
?
L-asparagine + H2O Pseudomonas sp. 7A
-
L-aspartate + NH3
-
?
L-glutamine + H2O Pseudomonas sp.
-
L-glutamate + NH3
-
?
L-glutamine + H2O Pseudomonas sp. 7A
-
L-glutamate + NH3
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. P10182
-
-
Pseudomonas sp. 7A P10182
-
-

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Pseudomonas sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-asparagine + H2O
-
Pseudomonas sp. D-aspartate + NH3
-
?
D-asparagine + H2O
-
Pseudomonas sp. 7A D-aspartate + NH3
-
?
D-glutamine + H2O
-
Pseudomonas sp. D-glutamate + NH3
-
?
D-glutamine + H2O
-
Pseudomonas sp. 7A D-glutamate + NH3
-
?
L-asparagine + H2O
-
Pseudomonas sp. L-aspartate + NH3
-
?
L-asparagine + H2O
-
Pseudomonas sp. 7A L-aspartate + NH3
-
?
L-glutamine + H2O
-
Pseudomonas sp. L-glutamate + NH3
-
?
L-glutamine + H2O
-
Pseudomonas sp. 7A L-glutamate + NH3
-
?

Subunits

Subunits Comment Organism
tetramer native crystallization Pseudomonas sp.