Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.1.33 extracted from

  • Mine, S.; Ikegami, T.; Kawasaki, K.; Nakamura, T.; Uegaki, K.
    Expression, refolding, and purification of active diacetylchitobiose deacetylase from Pyrococcus horikoshii (2012), Protein Expr. Purif., 84, 265-269.
    View publication on PubMed
Search for more literature for 3.5.1.33

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed as inclusion bodies in Escherichia coli Rosetta (DE3) pLys. The insoluble inclusion body is solubilized and reactivated through a refolding procedure Pyrococcus horikoshii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31595
-
 6 * 31595, calculated from sequence Pyrococcus horikoshii
174000
-
 gel filtration Pyrococcus horikoshii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-D-glucosamine + H2O Pyrococcus horikoshii the enzyme is involved in chitin catabolic pathway D-glucosamine + acetate
-
 ?
N-acetyl-D-glucosamine + H2O Pyrococcus horikoshii DSM 12428 the enzyme is involved in chitin catabolic pathway D-glucosamine + acetate
-
 ?

Organism

Organism UniProt Comment Textmining
Pyrococcus horikoshii O58235
-
-
Pyrococcus horikoshii DSM 12428 O58235
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pyrococcus horikoshii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-D-glucosamine + H2O
-
 Pyrococcus horikoshii D-glucosamine + acetate
-
 ?
N-acetyl-D-glucosamine + H2O the enzyme is involved in chitin catabolic pathway Pyrococcus horikoshii D-glucosamine + acetate
-
 ?
N-acetyl-D-glucosamine + H2O
-
 Pyrococcus horikoshii DSM 12428 D-glucosamine + acetate
-
 ?
N-acetyl-D-glucosamine + H2O the enzyme is involved in chitin catabolic pathway Pyrococcus horikoshii DSM 12428 D-glucosamine + acetate
-
 ?

Subunits

Subunits Comment Organism
homohexamer 6 * 31595, calculated from sequence Pyrococcus horikoshii

Synonyms

Synonyms Comment Organism
Ph-Dac
-
 Pyrococcus horikoshii
PH0499 locus name Pyrococcus horikoshii