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Literature summary for 3.5.1.32 extracted from
- Hippurathydrolase, ein neues Enzym aus Mikroorganismen, 2. Mitt.: Enzymeigenschaften (1968), Monatsh. Chem., 99, 2278-2290.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 8-hydroxyquinoline | 60% inhibition at 3.3 mM | Fusarium incarnatum |  |
| Ag2+ | complete inhibition at 1 mM | Fusarium incarnatum | |
| Ca2+ | slight inhibition at 1 mM | Fusarium incarnatum | |
| Co2+ | slight inhibition at 1 mM | Fusarium incarnatum | |
| Cu2+ | complete inhibition at 1 mM | Fusarium incarnatum | |
| EDTA | 40% inhibition at 10 mM | Fusarium incarnatum | |
| Fe2+ | - |
Fusarium incarnatum | |
| Hg2+ | 82% inhibition at 1 mM | Fusarium incarnatum | |
| iodoacetic acid | 20% inhibition at 10 mM | Fusarium incarnatum | |
| p-chloromercuribenzoic acid | complete inhibition at 1 mM | Fusarium incarnatum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0006 | - |
hippurate | at 5°C | Fusarium incarnatum | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Fusarium incarnatum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Fusarium incarnatum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | induction of enzyme synthesis by hippurate-containing media | Fusarium incarnatum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
- |
Fusarium incarnatum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 10-20% loss of activity within few weeks | Fusarium incarnatum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-benzoylglycine + H2O | - |
Fusarium incarnatum | benzoic acid + glycine | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Fusarium incarnatum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 5 | - |
60% inactivation after 24 h | Fusarium incarnatum |
| 60 | - |
rapid inactivation in absence of substrate | Fusarium incarnatum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Fusarium incarnatum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | - |
Fusarium incarnatum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.3 | - |
irreversible denaturation | Fusarium incarnatum |
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Release 2023.1 (January 2023)
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