Literature summary for 3.5.1.28 extracted from

Kerff, F.; Petrella, S.; Mercier, F.; Sauvage, E.; Herman, R.; Pennartz, A.; Zervosen, A.; Luxen, A.; Fr�re, J.; Joris, B.; Charlier, P.
Specific structural features of the N-acetylmuramoyl-L-alanine amidase amid from Escherichia coli and mechanistic implications for enzymes of this family (2010), J. Mol. Biol., 397, 249-259.

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli LMG194 and B180 cells	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, using 1 M LiCl, 10% (w/v) polyethylene glycol 6000 in a 0.1 M sodium citrate buffer (pH 4) for the native structure, and 1 M MgSO4 in a 0.1 M sodium acetate buffer (pH 4.6) for the Se-Met structure. The apoenzyme in complex with the substrate anhydro-N-acetylmuramic-acid-L-Ala-gamma-D-Glu-L-Lys is crystallized using 50% (w/v) polyethylene glycol 6000, 0.1 M sodium citrate buffer (pH 4), and 5 mM EDTA. The holoenzyme in complex with the product L-Ala-gamma-D-Glu-L-Lys is crystallized using 1 M MgCl2 in a 0.1 M sodium acetate buffer, pH 4.6	Escherichia coli

Crystallization (Comment)

Organism

hanging drop vapor diffusion method, using 1 M LiCl, 10% (w/v) polyethylene glycol 6000 in a 0.1 M sodium citrate buffer (pH 4) for the native structure, and 1 M MgSO4 in a 0.1 M sodium acetate buffer (pH 4.6) for the Se-Met structure. The apoenzyme in complex with the substrate anhydro-N-acetylmuramic-acid-L-Ala-gamma-D-Glu-L-Lys is crystallized using 50% (w/v) polyethylene glycol 6000, 0.1 M sodium citrate buffer (pH 4), and 5 mM EDTA. The holoenzyme in complex with the product L-Ala-gamma-D-Glu-L-Lys is crystallized using 1 M MgCl2 in a 0.1 M sodium acetate buffer, pH 4.6

Escherichia coli

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
periplasm	-	Escherichia coli	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	contains zinc	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P75820	-	-

Purification (Commentary)

Purification (Comment)	Organism
S-Sepharose column chromatography, Q-Sepharose column chromatography, and Sephacryl S100 gel filtration	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
1,6-anhydro-N-acetylmuramic-acid-L-Ala-gamma-D-Glu-L-Lys + H2O	-	Escherichia coli	1,6-anhydro-N-acetylmuramate + L-Ala-gamma-D-Glu-L-Lys	-	?
additional information	the enzyme has no activity on MurNAc-L-Ala-D-Glu	Escherichia coli	?	-	?
N-acetylmuramoyl-L-alanine + H2O	-	Escherichia coli	N-acetylmuramate + L-alanine	-	?

Subunits

Subunits	Comment	Organism
homodimer	-	Escherichia coli

Synonyms

Synonyms	Comment	Organism
AmiD	-	Escherichia coli