Cloned (Comment) | Organism |
---|---|
gene amiB, DNA and amino acid sequence determination and analysis, overexpression of the C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha | Pseudomonas aeruginosa |
overexpression in Escherichia coli | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pseudomonas aeruginosa | the enzyme cleaves the amide linkage between the stem peptides and the lactyl moiety of muramoyl residues in bacterial cell wall heteropolymer peptidoglycan | ? | - |
? | |
peptidoglycan + H2O | Pseudomonas aeruginosa | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Pseudomonas aeruginosa | - |
strain PA01, gene amiB | - |
Purification (Comment) | Organism |
---|---|
combination of affinity and cation-exchange chromatographies | Pseudomonas aeruginosa |
recombinant C-terminally His-tagged enzyme 38fold from Escherichia coli by nickel affinity and cation exchange chromatography to 98% homogeneity | Pseudomonas aeruginosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
132 | - |
purified recombinant enzyme | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme cleaves the amide linkage between the stem peptides and the lactyl moiety of muramoyl residues in bacterial cell wall heteropolymer peptidoglycan | Pseudomonas aeruginosa | ? | - |
? | |
peptidoglycan + H2O | - |
Pseudomonas aeruginosa | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AmiB | - |
Pseudomonas aeruginosa |
Autolysin | - |
Pseudomonas aeruginosa |
N-acetylmuramoyl-l-alanine amidase B | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Pseudomonas aeruginosa |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pseudomonas aeruginosa | AmiB, sequence calculation | - |
9.79 |