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Literature summary for 3.5.1.28 extracted from

  • Scheurwater, E.M.; Pfeffer, J.M.; Clarke, A.J.
    Production and purification of the bacterial autolysin N-acetylmuramoyl-l-alanine amidase B from Pseudomonas aeruginosa (2007), Protein Expr. Purif., 56, 128-137.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene amiB, DNA and amino acid sequence determination and analysis, overexpression of the C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha Pseudomonas aeruginosa
overexpression in Escherichia coli Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pseudomonas aeruginosa the enzyme cleaves the amide linkage between the stem peptides and the lactyl moiety of muramoyl residues in bacterial cell wall heteropolymer peptidoglycan ?
-
?
peptidoglycan + H2O Pseudomonas aeruginosa
-
?
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-
Pseudomonas aeruginosa
-
strain PA01, gene amiB
-

Purification (Commentary)

Purification (Comment) Organism
combination of affinity and cation-exchange chromatographies Pseudomonas aeruginosa
recombinant C-terminally His-tagged enzyme 38fold from Escherichia coli by nickel affinity and cation exchange chromatography to 98% homogeneity Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
132
-
purified recombinant enzyme Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme cleaves the amide linkage between the stem peptides and the lactyl moiety of muramoyl residues in bacterial cell wall heteropolymer peptidoglycan Pseudomonas aeruginosa ?
-
?
peptidoglycan + H2O
-
Pseudomonas aeruginosa ?
-
?

Synonyms

Synonyms Comment Organism
AmiB
-
Pseudomonas aeruginosa
Autolysin
-
Pseudomonas aeruginosa
N-acetylmuramoyl-l-alanine amidase B
-
Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
assay at Pseudomonas aeruginosa

pI Value

Organism Comment pI Value Maximum pI Value
Pseudomonas aeruginosa AmiB, sequence calculation
-
9.79