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Literature summary for 3.5.1.28 extracted from

  • Llull, D.; Lopez, R.; Garcia, E.
    Skl, a novel choline-binding N-acetylmuramoyl-L-alanine amidase of Streptococcus mitis SK137 containing a CHAP domain (2006), FEBS Lett., 580, 1959-1964.
    View publication on PubMed
Search for more literature for 3.5.1.28

Cloned(Commentary)

Cloned (Comment) Organism
gene skl, expression in Escherichia coli strain DH10B Streptococcus mitis

Organism

Organism UniProt Comment Textmining
Streptococcus mitis
-
 gene skl
-
Streptococcus mitis SK137
-
 gene skl
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.02
-
 Skl, substrate are choline-containing cell walls of Streptococcus mitis Streptococcus mitis
0.03
-
 Skl, substrate are choline-containing cell walls of Streptococcus pneumoniae Streptococcus mitis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme performs lysis of pneumococcal, choline-containing cell walls Streptococcus mitis ?
-
 ?
additional information the enzyme performs lysis of pneumococcal, choline-containing cell walls Streptococcus mitis SK137 ?
-
 ?

Subunits

Subunits Comment Organism
More the enzyme contains a cysteine, histidine-dependent amidohydrolase/peptidase domain, i.e. CHAP domain Streptococcus mitis

Synonyms

Synonyms Comment Organism
More the enzyme is a member of the choline-binding family, subfamily CHAP-containing enzymes, of proteins Streptococcus mitis
Skl
-
 Streptococcus mitis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Streptococcus mitis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Streptococcus mitis