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Literature summary for 3.5.1.28 extracted from
- Characterization of the N-acetylmuramic acid L-alanine amidase from Bacillus subtilis (1976), J. Bacteriol., 127, 803-811.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell wall | - |
Bacillus subtilis | 5618 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | absolute requirement for divalent cation, maximal activity with 0.02 M MgCl2 | Bacillus subtilis |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 51000 | - |
SDS-PAGE | Bacillus subtilis |
| 62000 | - |
sucrose density gradient centrifugation | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus subtilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 46.7 | - |
- |
Bacillus subtilis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, several months, no loss of activity | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cell walls | from Bacillus subtilis | Bacillus subtilis | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.8 | - |
- |
Bacillus subtilis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9.8 | - |
Bacillus subtilis |
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