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Literature summary for 3.5.1.26 extracted from

  • Kohno, M.; Yamashina, I.
    Purification and properties of 4-L-aspartylglycosylamine amidohydrolase from hog kidney (1972), Biochim. Biophys. Acta, 258, 600-617.
    View publication on PubMed
Search for more literature for 3.5.1.26

General Stability

General Stability Organism
stabilized by its substrate Asn-GlcNAc Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
aspartic acid 40% inhibition at 1 mM Sus scrofa
Cu2+
-
 Sus scrofa
EDTA 50% inhibition at 1 mM Sus scrofa
Mn2+
-
 Sus scrofa
Ni2+
-
 Sus scrofa
Zn2+
-
 Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
 2-acetamido-1-N-(4-L-aspartyl)-2-deoxy-beta-D-glycopyranosylamine for amidase-3 Sus scrofa
0.64
-
 2-acetamido-1-N-(4-L-aspartyl)-2-deoxy-beta-D-glycopyranosylamine amidase-1 and amidase-2, value depending on enzyme concentration Sus scrofa
0.77
-
 ovalbumin
-
 Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 disc electrophoresis, ultracentrifugation Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography techniques Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
kidney 3 forms Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.55
-
-
 Sus scrofa

Storage Stability

Storage Stability Organism
-20°C, 0.02 M phosphate buffer, pH 7.0, invariably stable Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-acetamido-1-beta-(L-aspartamido)-1,2-dideoxy-beta-D-glucose + H2O
-
 Sus scrofa 2-acetamido-1-amino-1,2-dideoxy-beta-D-glucose + L-aspartate additional product: NH3, in equimolar amounts of N-acetylglucosamine and L-aspartate ?
ovalbumin + H2O
-
 Sus scrofa L-aspartate + ?
-
 ?
Taka-amylase + H2O
-
 Sus scrofa L-aspartate + ?
-
 ?

Synonyms

Synonyms Comment Organism
amidase-1
-
 Sus scrofa
amidase-2
-
 Sus scrofa
amidase-3
-
 Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 sharp activity peak Sus scrofa
8
-
 considerable aciivity with a broad peak Sus scrofa

pH Stability

pH Stability pH Stability Maximum Comment Organism
6
-
 stable above Sus scrofa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1
-
 EDTA
-
 Sus scrofa