Literature summary for 3.5.1.25 extracted from

Choi, C.W.; An, H.Y.; Lee, Y.J.; Lee, Y.G.; Yun, S.H.; Park, E.C.; Hong, Y.; Kim, G.H.; Park, J.E.; Baek, S.J.; Kim, H.S.; Kim, S.I.
Characterization of Streptococcus pneumoniae N-acetylglucosamine-6-phosphate deacetylase as a novel diagnostic marker (2013), J. Microbiol., 51, 659-664.

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Application

Application	Comment	Organism
diagnostics	the enzyme has potential as a diagnostic marker for Streptococcus pneumoniae because of its high immunogenicity and immunospecificity	Streptococcus pneumoniae

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain C43(DE3)	Streptococcus pneumoniae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Streptococcus pneumoniae	5829	-
extracellular	-	Streptococcus pneumoniae	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
41670	-	x * 41670, sequence calculation, x * 45498, recombinant His-tagged enzyme, mass spectrometry	Streptococcus pneumoniae
45498	-	x * 41670, sequence calculation, x * 45498, recombinant His-tagged enzyme, mass spectrometry	Streptococcus pneumoniae

Organism

Organism	UniProt	Comment	Textmining
Streptococcus pneumoniae	Q8DN83	-	-
Streptococcus pneumoniae BAA-255	Q8DN83	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
additional information	the enzyme does not undergo posttranslational modification	Streptococcus pneumoniae

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme from Escherichia coli strain C43(DE3) by nickel affinity chromatography and dialysis	Streptococcus pneumoniae

Subunits

Subunits	Comment	Organism
?	x * 41670, sequence calculation, x * 45498, recombinant His-tagged enzyme, mass spectrometry	Streptococcus pneumoniae
More	the enzyme contains four internal disulfide bonds, Cys246, Cys256, Cys274, and Cys350 that may contribute to the formation of six internal disulfide bridges. Tryptic peptide mapping of the enzyme, overview	Streptococcus pneumoniae

Synonyms

Synonyms	Comment	Organism
NagA	-	Streptococcus pneumoniae

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Release 2023.1 (January 2023)