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Literature summary for 3.5.1.25 extracted from
- N-Acetylglucosamine-6-phosphate deacetylase from Escherichia coli: purification and molecular and kinetic characterization (1997), Arch. Biochem. Biophys., 340, 338-346.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,5'-dithio-bis(2-nitrobenzoate) | - |
Escherichia coli |  |
| acetate | competitive | Escherichia coli | |
| N-acetyl-D-glucosamine 6-phosphate | substrate inhibition at high concentrations | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.3 | - |
N-acetyl-D-glucosamine 6-phosphate | - |
Escherichia coli | |
| 20 | - |
acetate | - |
Escherichia coli | |
| 25 | - |
glucosamine 6-phosphate | - |
Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41000 | - |
4 * 41000, SDS-PAGE | Escherichia coli |
| 160000 | 165000 | non-denaturing PAGE, gel filtration | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
overproducing strain | - |
| Escherichia coli overproducing | - |
overproducing strain | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| N-acetyl-D-glucosamine 6-phosphate + H2O = D-glucosamine 6-phosphate + acetate | sequential mechanism with ordered release of products and a slow isomerization of the enzyme-acetate complex | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucosamine 6-phosphate + acetate | - |
Escherichia coli | N-acetyl-D-glucosamine 6-phosphate + H2O | - |
r | |
| D-glucosamine 6-phosphate + acetate | - |
Escherichia coli overproducing | N-acetyl-D-glucosamine 6-phosphate + H2O | - |
r | |
| N-acetyl-D-glucosamine 6-phosphate + H2O | - |
Escherichia coli | D-glucosamine 6-phosphate + acetate | - |
r | |
| N-acetyl-D-glucosamine 6-phosphate + H2O | - |
Escherichia coli overproducing | D-glucosamine 6-phosphate + acetate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 41000, SDS-PAGE | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 105 | - |
N-acetyl-D-glucosamine 6-phosphate | - |
Escherichia coli | |
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