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Literature summary for 3.5.1.24 extracted from

  • Kumar, R.; Rajkumar, H.; Kumar, M.; Varikuti, S.R.; Athimamula, R.; Shujauddin, M.; Ramagoni, R.; Kondapalli, N.
    Molecular cloning, characterization and heterologous expression of bile salt hydrolase (Bsh) from Lactobacillus fermentum NCDO394 (2013), Mol. Biol. Rep., 40, 5057-5066.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene bsh, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) Limosilactobacillus fermentum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36490
-
x * 36490, sequence calculation, x * 36500, recombinant His6-tagged enzyme, SDS-PAGE Limosilactobacillus fermentum
36500
-
x * 36490, sequence calculation, x * 36500, recombinant His6-tagged enzyme, SDS-PAGE Limosilactobacillus fermentum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glycocholate + H2O Limosilactobacillus fermentum preferred substrate cholate + glycine
-
?
glycocholate + H2O Limosilactobacillus fermentum NCDO394 preferred substrate cholate + glycine
-
?

Organism

Organism UniProt Comment Textmining
Limosilactobacillus fermentum H6WSA2 gene bsh
-
Limosilactobacillus fermentum NCDO394 H6WSA2 gene bsh
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nicke affinity chromatography to homogeneity Limosilactobacillus fermentum

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycocholate + H2O preferred substrate Limosilactobacillus fermentum cholate + glycine
-
?
glycocholate + H2O preferred substrate Limosilactobacillus fermentum NCDO394 cholate + glycine
-
?
additional information the recombinant enzyme hydrolyzes six major human bile salts with a slight preference towards glycine-conjugated bile salts, the enzyme shows a broad range of substrate specificity, overview. The enzyme also slightly prefers to hydrolyze conjugated cholates over conjugated deoxycholates and chenodeoxycholates Limosilactobacillus fermentum ?
-
?
additional information the recombinant enzyme hydrolyzes six major human bile salts with a slight preference towards glycine-conjugated bile salts, the enzyme shows a broad range of substrate specificity, overview. The enzyme also slightly prefers to hydrolyze conjugated cholates over conjugated deoxycholates and chenodeoxycholates Limosilactobacillus fermentum NCDO394 ?
-
?

Subunits

Subunits Comment Organism
? x * 36490, sequence calculation, x * 36500, recombinant His6-tagged enzyme, SDS-PAGE Limosilactobacillus fermentum

Synonyms

Synonyms Comment Organism
bile salt hydrolase
-
Limosilactobacillus fermentum
BSH
-
Limosilactobacillus fermentum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
recombinant enzyme Limosilactobacillus fermentum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 65 purified recombinant enzyme, stable at Limosilactobacillus fermentum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Limosilactobacillus fermentum

pH Range

pH Minimum pH Maximum Comment Organism
5 7 the enzyme activity sharply declines below pH 5.0 and above pH 7.0 Limosilactobacillus fermentum

pI Value

Organism Comment pI Value Maximum pI Value
Limosilactobacillus fermentum sequence calculation
-
6.39