Cloned (Comment) | Organism |
---|---|
gene cgh, DNA and amino acid sequence determination and analysis, sequence comparisons, expression in Escherichia coli strain BL21(DE3) | Brucella abortus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of an isogenic deletion mutant strain DELTAcgh, the growth of the the cgh mutant is inhibited by addition of 5% or 10% bile acid, but is similar to the wild-type strain in absence of bile acids, the mutant enzyme does not confer resistance to bile acids to the organism | Brucella abortus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Brucella abortus | the enzyme contributes to the establishment of a successful infection through the oral route in mice, CGH confers the ability to the organism to resist the antimicrobial action of bile salts | ? | - |
? | |
additional information | Brucella abortus 2308 | the enzyme contributes to the establishment of a successful infection through the oral route in mice, CGH confers the ability to the organism to resist the antimicrobial action of bile salts | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brucella abortus | Q2YS20 | - |
- |
Brucella abortus 2308 | Q2YS20 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain BL21(DE3) by anion exchange chromatography, and polymyxin B resin chromatography, the latter to eliminate lipopolysaccharide contamination | Brucella abortus |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycocholate + H2O | - |
Brucella abortus | glycine + cholate | - |
? | |
glycocholate + H2O | - |
Brucella abortus 2308 | glycine + cholate | - |
? | |
additional information | the enzyme contributes to the establishment of a successful infection through the oral route in mice, CGH confers the ability to the organism to resist the antimicrobial action of bile salts | Brucella abortus | ? | - |
? | |
additional information | the enzyme contributes to the establishment of a successful infection through the oral route in mice, CGH confers the ability to the organism to resist the antimicrobial action of bile salts | Brucella abortus 2308 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
bile salt hydrolase | - |
Brucella abortus |
CGH | - |
Brucella abortus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Brucella abortus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Brucella abortus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | activity range | Brucella abortus |