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Literature summary for 3.5.1.19 extracted from

  • Gazanion, E.; Garcia, D.; Silvestre, R.; Gerard, C.; Guichou, J.F.; Labesse, G.; Seveno, M.; Cordeiro-Da-Silva, A.; Ouaissi, A.; Sereno, D.; Vergnes, B.
    The Leishmania nicotinamidase is essential for NAD+ production and parasite proliferation (2011), Mol. Microbiol., 82, 21-38.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Leishmania infantum

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting-drop-based and sparse-matrix screening , using 0.7 M Na-citrate and 0.1 M HEPES (pH 7.5) Leishmania infantum

Protein Variants

Protein Variants Comment Organism
C161A inactive Leishmania infantum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol under physiological conditions, the enzyme displays a diffuse, mainly cytosolic localization Leishmania infantum 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22900
-
x * 22900, calculated from amino acid sequence Leishmania infantum
24000
-
x * 24000, SDS-PAGE Leishmania infantum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nicotinamide + H2O Leishmania infantum
-
nicotinate + NH3
-
?
nicotinamide + H2O Leishmania infantum MHOM/MA/67/ITMAP-263
-
nicotinate + NH3
-
?
pyrazinamide + H2O Leishmania infantum
-
pyrazine-2-carboxylic acid + NH3
-
?
pyrazinamide + H2O Leishmania infantum MHOM/MA/67/ITMAP-263
-
pyrazine-2-carboxylic acid + NH3
-
?

Organism

Organism UniProt Comment Textmining
Leishmania infantum
-
-
-
Leishmania infantum MHOM/MA/67/ITMAP-263
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Leishmania infantum

Source Tissue

Source Tissue Comment Organism Textmining
promastigote
-
Leishmania infantum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nicotinamide + H2O
-
Leishmania infantum nicotinate + NH3
-
?
nicotinamide + H2O
-
Leishmania infantum MHOM/MA/67/ITMAP-263 nicotinate + NH3
-
?
pyrazinamide + H2O
-
Leishmania infantum pyrazine-2-carboxylic acid + NH3
-
?
pyrazinamide + H2O
-
Leishmania infantum MHOM/MA/67/ITMAP-263 pyrazine-2-carboxylic acid + NH3
-
?

Subunits

Subunits Comment Organism
? x * 24000, SDS-PAGE Leishmania infantum
? x * 22900, calculated from amino acid sequence Leishmania infantum

Synonyms

Synonyms Comment Organism
Pnc1
-
Leishmania infantum

General Information

General Information Comment Organism
malfunction the generation of nicotinamidase null mutants leads to a decrease in NAD+ content, associated with a metabolic shutdown-like phenotype with an extensive lag phase of growth. The null mutants are also unable to establish a sustained infection in a mouse model Leishmania infantum
physiological function Leishmania nicotinamidase is essential for NAD+ production and parasite proliferation Leishmania infantum