Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
H57D | the mutant has reduced Mn2+ content and shows a 6fold and 38fold decrease in kcat value for nicotinamide and pyrazinamide, respectively | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-pyridine carboxaldehyde | competitive inhibitor | Mycobacterium tuberculosis | |
iodoacetamide | reversible, competitive inhibitor | Mycobacterium tuberculosis | |
pyrazinecarbonitrile | irreversible inactivator | Mycobacterium tuberculosis | |
pyridine-3-carbonitrile | reversible, competitive inhibitor | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
nicotinamide | mutant enzyme H57D, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis | |
0.014 | - |
nicotinamide | wild type enzyme, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis | |
0.016 | - |
Pyrazinamide | mutant enzyme H57D, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis | |
0.3 | - |
Pyrazinamide | wild type enzyme, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | the enzyme contains 0.47 M of Fe2+ per mol of enzyme | Mycobacterium tuberculosis | |
Mn2+ | the enzyme contains 0.44 M Mn2+ per mol of enzyme | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nicotinamide + H2O | Mycobacterium tuberculosis | - |
nicotinate + NH3 | - |
? | |
nicotinamide + H2O | Mycobacterium tuberculosis H37Rv | - |
nicotinate + NH3 | - |
? | |
pyrazinamide + H2O | Mycobacterium tuberculosis | - |
pyrazine-2-carboxylic acid + NH3 | - |
? | |
pyrazinamide + H2O | Mycobacterium tuberculosis H37Rv | - |
pyrazine-2-carboxylic acid + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Mycobacterium tuberculosis H37Rv | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nicotinamide + H2O | - |
Mycobacterium tuberculosis | nicotinate + NH3 | - |
? | |
nicotinamide + H2O | - |
Mycobacterium tuberculosis H37Rv | nicotinate + NH3 | - |
? | |
pyrazinamide + H2O | - |
Mycobacterium tuberculosis | pyrazine-2-carboxylic acid + NH3 | - |
? | |
pyrazinamide + H2O | - |
Mycobacterium tuberculosis H37Rv | pyrazine-2-carboxylic acid + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
nicotinamidase/pyrazinamidase | - |
Mycobacterium tuberculosis |
PncA | - |
Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
Pyrazinamide | mutant enzyme H57D, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis | |
0.5 | - |
nicotinamide | mutant enzyme H57D, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis | |
3.1 | - |
nicotinamide | wild type enzyme, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis | |
3.8 | - |
Pyrazinamide | wild type enzyme, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
around pH 8.0 | Mycobacterium tuberculosis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00029 | - |
3-pyridine carboxaldehyde | wild type enzyme, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis | |
0.1 | - |
pyridine-3-carbonitrile | Ki about 0.1 mM, wild type enzyme, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the NAD+ salvage pathway | Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.2 | - |
Pyrazinamide | mutant enzyme H57D, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis | |
13 | - |
Pyrazinamide | wild type enzyme, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis | |
37 | - |
nicotinamide | mutant enzyme H57D, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis | |
220 | - |
nicotinamide | wild type enzyme, in 100 mM HEPES at pH 8.0 and 25°C | Mycobacterium tuberculosis |