Literature summary for 3.5.1.19 extracted from

Hu, G.; Taylor, A.B.; McAlister-Henn, L.; Hart, P.J.
Crystal structure of the yeast nicotinamidase Pnc1p (2007), Arch. Biochem. Biophys., 461, 66-75.

Search for more literature for 3.5.1.19

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Saccharomyces cerevisiae

Crystallization (Commentary)

Crystallization (Comment)	Organism
resolution 2.9 Angstrom	Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.21	-	nicotinamide	about 3fold higher than with pyrazinamide as substrate	Saccharomyces cerevisiae

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	-	Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
nicotinamide + H2O	Saccharomyces cerevisiae	-	NH3 + nicotinic acid	-	?
pyrazinamide + H2O	Saccharomyces cerevisiae	-	NH3 + pyrazinoic acid	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	P53184	-	-

Purification (Commentary)

Purification (Comment)	Organism
immobilized metal affinity chromatography	Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
50.2	-	-	Saccharomyces cerevisiae
59.4	-	-	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
nicotinamide + H2O	-	Saccharomyces cerevisiae	NH3 + nicotinic acid	-	?
pyrazinamide + H2O	-	Saccharomyces cerevisiae	NH3 + pyrazinoic acid	-	?

Synonyms

Synonyms	Comment	Organism
nicotinamidase Pnc1p	-	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)