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Literature summary for 3.5.1.19 extracted from
- Nicotinamide deamidase from Flavobacterium peregrinum (1980), Methods Enzymol., 66, 132-136.
General Stability
| General Stability | Organism |
|---|---|
| inactivated by dialysis in absence of Mn2+. After overnight dialysis in presence of Mn2+ almost all activity is retained. A dialysis against 0.01 M maleate buffer, pH 6.5, containing 0.005 mM HgCl2 results in complete retention of activity | Flavobacterium peregrinum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3-acetylpyridine | competitive | Flavobacterium peregrinum |  |
| Cu2+ | CuSO4 | Flavobacterium peregrinum | |
| EDTA | - |
Flavobacterium peregrinum | |
| Fe2+ | FeCl2 | Flavobacterium peregrinum | |
| HgCl2 | - |
Flavobacterium peregrinum | |
| N,N'-diethylnicotinamide | competitive | Flavobacterium peregrinum | |
| N2-ethylnicotinamide | competitive | Flavobacterium peregrinum | |
| N2-methylnicotinamide | competitive | Flavobacterium peregrinum | |
| nicotinic acid hydrazide | competitive | Flavobacterium peregrinum | |
| PCMB | - |
Flavobacterium peregrinum | |
| Zn2+ | ZnCl2 | Flavobacterium peregrinum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0002 | - |
nicotinamide | - |
Flavobacterium peregrinum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
gel filtration | Flavobacterium peregrinum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Flavobacterium peregrinum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Flavobacterium peregrinum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.17 | - |
- |
Flavobacterium peregrinum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 50% loss of activity after 4 weeks | Flavobacterium peregrinum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nicotinamide + H2O | - |
Flavobacterium peregrinum | nicotinate + NH3 | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Flavobacterium peregrinum |
| 6.6 | - |
- |
Flavobacterium peregrinum |
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Release 2023.1 (January 2023)
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