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Literature summary for 3.5.1.12 extracted from

  • Chauhan, J.; Dakshinamurti, K.
    Purification and characterization of human serum biotinidase (1986), J. Biol. Chem., 261, 4268-4275.
    View publication on PubMed
Search for more literature for 3.5.1.12

Application

Application Comment Organism
medicine plasma biotinidase activity assay for diagnosis and screen newborn infants for biotinidase deficiency, treatment of inborn metabolism errors with large doses of biotin Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
biotin competitive inhibition at acidic pH, no inhibition at ph 7.4 with p-(N-biotinylamino)benzoic acid as substrate Enterococcus faecalis
biotin competitive inhibition at acidic pH, no inhibition at ph 7.4 with p-(N-biotinylamino)benzoic acid as substrate Homo sapiens
biotin competitive inhibition at acidic pH, no inhibition at ph 7.4 with p-(N-biotinylamino)benzoic acid as substrate Sus scrofa
CuCl2
-
 Homo sapiens
CuCl2
-
 Rattus rattus
guanidine hydrochloride 0.5 M Homo sapiens
Urea 0.5 M Homo sapiens
ZnCl2
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
 epsilon-N-biotinyl-L-lysine human serum Homo sapiens
0.0065
-
 epsilon-N-biotinyl-L-lysine human plasma Homo sapiens
0.0077
-
 epsilon-N-biotinyl-L-lysine pH 5.5 Homo sapiens
0.0078
-
 epsilon-N-biotinyl-L-lysine pH 7 Homo sapiens
0.008
-
 epsilon-N-biotinyl-L-lysine serum Sus scrofa
0.01
-
 epsilon-N-biotinyl-L-lysine porcine liver Sus scrofa
0.016
-
 epsilon-N-biotinyl-L-lysine kidney Sus scrofa
0.0306
-
 p-(N-biotinylamino)benzoic acid pH 5.5, D-biotin as inhibitor Homo sapiens
0.0319
-
 p-(N-biotinylamino)benzoic acid pH 5.5 Homo sapiens
0.0333
-
 p-(N-biotinylamino)benzoic acid pH 7 Homo sapiens
0.0341
-
 p-(N-biotinylamino)benzoic acid pH 7, D-biocytin as inhibitor Homo sapiens
0.0685
-
 N-DL-desthiobiotinyl-p-aminobenzoic acid pH 7 Homo sapiens
0.0714
-
 N-DL-desthiobiotinyl-p-aminobenzoic acid pH 5.5 Homo sapiens
0.0905
-
 m-(N-biotinylamino)benzoic acid pH 5.5 Homo sapiens
0.1002
-
 m-(N-biotinylamino)benzoic acid
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Homo sapiens 5739
-
mitochondrion
-
 Rattus rattus 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
-
 Sus scrofa
68000
-
 gel filtration, SDS-PAGE, sedimentation analysis Homo sapiens
76000
-
-
 Homo sapiens
76000
-
 gel filtration, SDS-PAGE Sus scrofa
80000
-
 SDS-PAGE Homo sapiens
110000
-
 gel filtration Homo sapiens
115000
-
-
 Homo sapiens
115000
-
 gel filtration Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
biotin amide + H2O Homo sapiens
-
 ?
-
 ?
biotin amide + H2O Sus scrofa
-
 ?
-
 ?
biotin amide + H2O Enterococcus faecalis
-
 ?
-
 ?
biotin amide + H2O Rattus rattus
-
 ?
-
 ?
epsilon-N-biotinyl-L-lysine + H2O Homo sapiens
-
 ?
-
 ?
epsilon-N-biotinyl-L-lysine + H2O Sus scrofa
-
 ?
-
 ?
epsilon-N-biotinyl-L-lysine + H2O Enterococcus faecalis
-
 ?
-
 ?
epsilon-N-biotinyl-L-lysine + H2O Rattus rattus
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Enterococcus faecalis
-
-
-
Homo sapiens
-
 human
-
Rattus rattus
-
 rat
-
Sus scrofa
-
 pig
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens
-
 Enterococcus faecalis

Source Tissue

Source Tissue Comment Organism Textmining
blood serum, plasma, leukocytes Homo sapiens
-
blood serum, plasma, leukocytes Sus scrofa
-
blood serum, plasma, leukocytes Rattus rattus
-
liver
-
 Homo sapiens
-
liver
-
 Sus scrofa
-
liver
-
 Rattus rattus
-
small intestine
-
 Homo sapiens
-
small intestine
-
 Rattus rattus
-
stomach
-
 Rattus rattus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
biotin amide + H2O
-
 Homo sapiens ?
-
 ?
biotin amide + H2O
-
 Sus scrofa ?
-
 ?
biotin amide + H2O
-
 Enterococcus faecalis ?
-
 ?
biotin amide + H2O
-
 Rattus rattus ?
-
 ?
epsilon-N-biotinyl-L-lysine + H2O
-
 Homo sapiens biotin + L-lysine
-
 ir
epsilon-N-biotinyl-L-lysine + H2O
-
 Sus scrofa biotin + L-lysine
-
 ir
epsilon-N-biotinyl-L-lysine + H2O
-
 Enterococcus faecalis biotin + L-lysine
-
 ir
epsilon-N-biotinyl-L-lysine + H2O
-
 Rattus rattus biotin + L-lysine
-
 ir
epsilon-N-biotinyl-L-lysine + H2O
-
 Homo sapiens ?
-
 ?
epsilon-N-biotinyl-L-lysine + H2O
-
 Sus scrofa ?
-
 ?
epsilon-N-biotinyl-L-lysine + H2O
-
 Enterococcus faecalis ?
-
 ?
epsilon-N-biotinyl-L-lysine + H2O
-
 Rattus rattus ?
-
 ?
N-(+)biotinyl-4-aminobenzoic acid + H2O
-
 Homo sapiens 4-aminobenzoic acid + biotin
-
 ?
N-biotinyl-3-aminobenzoic acid + H2O
-
 Homo sapiens biotin + 3-aminobenzoic acid
-
 ?
N-biotinyl-3-aminobenzoic acid + H2O
-
 Sus scrofa biotin + 3-aminobenzoic acid
-
 ?
N-DL-dethiobiotinyl-p-aminobenzoic acid + H2O
-
 Homo sapiens dethiobiotin + 4-aminobenzoic acid
-
 ?
p-(N-biotinylamino)benzoic acid + H2O
-
 Homo sapiens biotin + p-aminobenzoic acid
-
 ir
p-(N-biotinylamino)benzoic acid + H2O
-
 Sus scrofa biotin + p-aminobenzoic acid
-
 ir
p-(N-biotinylamino)benzoic acid + H2O
-
 Enterococcus faecalis biotin + p-aminobenzoic acid
-
 ir
p-(N-biotinylamino)benzoic acid + H2O
-
 Rattus rattus biotin + p-aminobenzoic acid
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 68000, SDS-PAGE Homo sapiens
monomer 1 * 68000, SDS-PAGE Sus scrofa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60 70
-
 Sus scrofa
60 70
-
 Rattus rattus
60 70 15 min 60°C 60% activity lost, 70°C activity completely lost Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5 6 with natural substrate biocytin Homo sapiens
6 7.5 with synthetic substrates Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 7
-
 Homo sapiens