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Literature summary for 3.5.1.11 extracted from

  • Orr, V.; Scharer, J.; Moo-Young, M.; Honeyman, C.; Fenner, D.; Crossley, L.; Suen, S.; Chou, C.
    Integrated development of an effective bioprocess for extracellular production of penicillin G acylase in Escherichia coli and its subsequent one-step purification (2012), J. Biotechnol., 161, 19-26.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene pac, overexpression in Escherichia coli strain HB101 and secretion of the enzyme to the culture medium, method optimization, overview Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Escherichia coli
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
penicillin G + H2O Escherichia coli
-
phenylacetate + 6-aminopenicillanate
-
?
penicillin G + H2O Escherichia coli JE5505
-
phenylacetate + 6-aminopenicillanate
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli JE5505
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant extracellular enzyme 3.2fold from Escherichia coli strain HB101 culture supernatant by anion exchange chromatography, single-step purification Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
16.3
-
purified recombinant enzyme, pH 8.0, 37°C Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
penicillin G + H2O
-
Escherichia coli phenylacetate + 6-aminopenicillanate
-
?
penicillin G + H2O
-
Escherichia coli JE5505 phenylacetate + 6-aminopenicillanate
-
?

Synonyms

Synonyms Comment Organism
PAC
-
Escherichia coli
penicillin G acylase
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Escherichia coli