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Literature summary for 3.5.1.11 extracted from

  • Pchelintsev, N.; Youshko, M.; Švedas, V.
    Quantitative characteristic of the catalytic properties and microstructure of cross-linked enzyme aggregates of penicillin acylase (2009), J. Mol. Catal. B, 56, 202-207.
No PubMed abstract available
Search for more literature for 3.5.1.11

Protein Variants

Protein Variants Comment Organism
additional information immobilization of the enzyme, quantitative characteristic of the catalytic properties and microstructure of cross-linked enzyme aggregates of penicillin acylase under different conditions by confocal fluorescent microscopy, overview. Comparison of fresh aggregates and mature aggregates. The aggregate size might regulate the extent of covalent modification of the enzyme and thereby influence the catalytic properties of cross-linked enzyme aggregates Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
PMSF irreversible, used for active site titrations of the cross-linked enzyme aggregates Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
 5-nitro-3-[(phenylacetyl)amino]benzoic acid pH 7.5, 25°C, native enzyme Escherichia coli
0.14
-
 5-nitro-3-[(phenylacetyl)amino]benzoic acid pH 7.5, 25°C, fresh immobilized cross-linked enzyme aggregates Escherichia coli
0.23
-
 5-nitro-3-[(phenylacetyl)amino]benzoic acid pH 7.5, 25°C, mature immobilized cross-linked enzyme aggregates Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-nitro-3-[(phenylacetyl)amino]benzoic acid + H2O
-
 Escherichia coli phenylacetate + 3-amino-5-nitro-benzoic acid
-
 ?
penicillin G + H2O
-
 Escherichia coli 6-aminopenicillanic acid + phenyl acetic acid
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.9
-
 5-nitro-3-[(phenylacetyl)amino]benzoic acid pH 7.5, 25°C, fresh immobilized cross-linked enzyme aggregates Escherichia coli
1
-
 5-nitro-3-[(phenylacetyl)amino]benzoic acid pH 7.5, 25°C, mature immobilized cross-linked enzyme aggregates Escherichia coli
1.2
-
 5-nitro-3-[(phenylacetyl)amino]benzoic acid pH 7.5, 25°C, native enzyme Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5 assay at Escherichia coli