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Literature summary for 3.5.1.11 extracted from

  • Kang, J.H.; Hwang, Y.; Yoo, O.J.
    Expression of penicillin G acylase gene from Bacillus megaterium ATCC 14945 in Escherichia coli and Bacillus subtilis (1991), J. Biotechnol., 17, 99-108.
    View publication on PubMed
Search for more literature for 3.5.1.11

Application

Application Comment Organism
synthesis production of 6-aminopenicillanate as precursor for the production of semi-synthetic beta-lactam derivatives Priestia megaterium

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli and Bacillus subtilis, high expression rate of extracellular enzyme in Bacillus subtilis Priestia megaterium

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Priestia megaterium
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
 2 * 57000, SDS-PAGE, native mass by gel filtration Priestia megaterium
115000
-
 gel filtration Priestia megaterium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
penicillin G + H2O Priestia megaterium
-
 6-aminopenicillanic acid + phenylacetic acid
-
 ?

Organism

Organism UniProt Comment Textmining
Priestia megaterium
-
 strain ATCC 14945
-

Purification (Commentary)

Purification (Comment) Organism
from cell culture medium of transgenic Bacillus subtilis Priestia megaterium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.19
-
 ampicillin as subsrate, purified enzyme, 37°C, pH 6.8 Priestia megaterium
0.23
-
 cephradine as subsrate, purified enzyme, 37°C, pH 6.8 Priestia megaterium
0.56
-
 cephalexin as subsrate, purified enzyme, 37°C, pH 6.8 Priestia megaterium
0.67
-
 cephaloglycin as subsrate, purified enzyme, 37°C, pH 6.8 Priestia megaterium
1.24
-
 cephaloridine as subsrate, purified enzyme, 37°C, pH 6.8 Priestia megaterium
1.36
-
 cefamandole as subsrate, purified enzyme, 37°C, pH 6.8 Priestia megaterium
1.58
-
 cephalothin as subsrate, purified enzyme, 37°C, pH 6.8 Priestia megaterium
1.86
-
 penicillin G as subsrate, purified enzyme, 37°C, pH 6.8 Priestia megaterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7-amino-desacetoxycephalosporanic acid + D-alpha-phenylglycine methylester acylating activity Priestia megaterium ?
-
 ?
7-aminocephalosporanic acid + D-alpha-phenylglycine methylester acylating activity Priestia megaterium ?
-
 ?
cefamandole + H2O
-
 Priestia megaterium (6R,7R)-7-amino-3-{[(1-methyl-1H-tetrazol-5-yl)sulfanyl]methyl}-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid + (2R)-hydroxy(phenyl)acetic acid
-
 ?
cephalexin + H2O
-
 Priestia megaterium (2R)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5-methyl-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
cephaloglycin + H2O
-
 Priestia megaterium ?
-
 ?
cephaloridine + H2O
-
 Priestia megaterium (6R,7R)-7-amino-8-oxo-3-[(pyridin-1-ium-1-yl)methyl]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylate + (thiophen-2-yl)acetic acid
-
 ?
cephalothin + H2O
-
 Priestia megaterium (6R,7R)-3-[(acetyloxy)methyl]-7-amino-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid + (thiophen-2-yl)acetic acid
-
 ?
cephradine + H2O
-
 Priestia megaterium (2R)-amino(cyclohexa-1,4-dien-1-yl)acetic acid + (6R,7R)-7-amino-3-methyl-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid
-
 ?
additional information no activity with cephapirin, cefotaximen cephalosporin, and penicillin N Priestia megaterium ?
-
 ?
penicillin G + H2O
-
 Priestia megaterium 6-aminopenicillanic acid + phenylacetic acid
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 57000, SDS-PAGE, native mass by gel filtration Priestia megaterium

Synonyms

Synonyms Comment Organism
penicillin G acylase
-
 Priestia megaterium
PGA
-
 Priestia megaterium