Application | Comment | Organism |
---|---|---|
synthesis | production of 6-aminopenicillanate and 7-amino-desacetoxycephalosporanic acid as precursor for the production of semi-synthetic beta-lactam derivatives | Priestia megaterium |
Cloned (Comment) | Organism |
---|---|
native and mutant proteins expresses in Bacillus subtilis WB600 | Priestia megaterium |
Protein Variants | Comment | Organism |
---|---|---|
F145A | alpha chain, no formation of cephalexin, no activity with nitrophenylacetylaminobenzoic acid | Priestia megaterium |
F145L | alpha chain, no formation of cephalexin, no activity with nitrophenylacetylaminobenzoic acid | Priestia megaterium |
F145Y | alpha chain, slightly increased formation of cephalexin, 32% of nitrophenylacetylaminobenzoic acid hydrolyzing activity | Priestia megaterium |
V24F | beta chain, no effect on the formation of cephalexin, 11% of nitrophenylacetylaminobenzoic acid hydrolyzing activity | Priestia megaterium |
Y144R | alpha chain, reduced formation of cephalexin, 72% of nitrophenylacetylaminobenzoic acid hydrolyzing activity | Priestia megaterium |
Y144R/V24F | Y144R on alpha chain, V24F on beta chain, reduced formation of cephalexin, 4% of nitrophenylacetylaminobenzoic acid hydrolyzing activity | Priestia megaterium |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Priestia megaterium | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
penicillin G + H2O | Priestia megaterium | - |
6-aminopenicillanic acid + phenylacetic acid | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Priestia megaterium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native and mutant proteins | Priestia megaterium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.01 | - |
nitrophenylacetylaminobenzoic acid as substrate, purified F145A mutant enzyme, 37°C | Priestia megaterium |
0.04 | - |
nitrophenylacetylaminobenzoic acid as substrate, purified F145L mutant enzyme, 37°C | Priestia megaterium |
0.85 | - |
7-amino-desacetoxycephalosporanic acid + D-phenylglycine amide as substrates, purified Y144R mutant enzyme, 30°C, pH 7.0 | Priestia megaterium |
0.904 | - |
7-amino-desacetoxycephalosporanic acid + D-phenylglycine amide as substrates, purified Y144R/V24F mutant enzyme, 30°C, pH 7.0 | Priestia megaterium |
1.22 | - |
nitrophenylacetylaminobenzoic acid as substrate, purified Y144R/V24F mutant enzyme, 37°C | Priestia megaterium |
1.48 | - |
7-amino-desacetoxycephalosporanic acid + D-phenylglycine amide as substrates, purified V24F mutant enzyme, 30°C, pH 7.0 | Priestia megaterium |
1.49 | - |
7-amino-desacetoxycephalosporanic acid + D-phenylglycine amide as substrates, purified native enzyme, 30°C, pH 7.0 | Priestia megaterium |
1.94 | - |
7-amino-desacetoxycephalosporanic acid + D-phenylglycine amide as substrates, purified F145Y mutant enzyme, 30°C, pH 7.0 | Priestia megaterium |
3.35 | - |
nitrophenylacetylaminobenzoic acid as substrate, purified V24F mutant enzyme, 37°C | Priestia megaterium |
9.73 | - |
nitrophenylacetylaminobenzoic acid as substrate, purified F145Y mutant enzyme, 37°C | Priestia megaterium |
22.1 | - |
nitrophenylacetylaminobenzoic acid as substrate, purified Y144R mutant enzyme, 37°C | Priestia megaterium |
30.6 | - |
nitrophenylacetylaminobenzoic acid as substrate, purified native enzyme, 37°C | Priestia megaterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-nitro-3-(phenylacetamido)benzoic acid + H2O | - |
Priestia megaterium | phenylacetic acid + 5-amino-2-nitrobenzoic acid | - |
? | |
7-amino-desacetoxycephalosporanic acid + D-phenylglycine amide | high reactant concentrations | Priestia megaterium | cephalexin | - |
r | |
cephalosporin G + H2O | - |
Priestia megaterium | 7-amino-desacetoxycephalosporanic acid + phenylacetic acid | - |
? | |
penicillin G + H2O | - |
Priestia megaterium | 6-aminopenicillanic acid + phenylacetic acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | alpha and beta chain | Priestia megaterium |
Synonyms | Comment | Organism |
---|---|---|
BmPGA | - |
Priestia megaterium |
penicillin G acylase | - |
Priestia megaterium |