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Literature summary for 3.5.1.11 extracted from

  • Wang, J.; Zhang, Q.; Huang, H.; Yuan, Z.; Ding, D.; Yang, S.; Jiang, W.
    Increasing synthetic performance of penicillin G acylase from Bacillus megaterium by site-directed mutagenesis (2007), Appl. Microbiol. Biotechnol., 74, 1023-1030.
    View publication on PubMed

Application

Application Comment Organism
synthesis production of 6-aminopenicillanate and 7-amino-desacetoxycephalosporanic acid as precursor for the production of semi-synthetic beta-lactam derivatives Priestia megaterium

Cloned(Commentary)

Cloned (Comment) Organism
native and mutant proteins expresses in Bacillus subtilis WB600 Priestia megaterium

Protein Variants

Protein Variants Comment Organism
F145A alpha chain, no formation of cephalexin, no activity with nitrophenylacetylaminobenzoic acid Priestia megaterium
F145L alpha chain, no formation of cephalexin, no activity with nitrophenylacetylaminobenzoic acid Priestia megaterium
F145Y alpha chain, slightly increased formation of cephalexin, 32% of nitrophenylacetylaminobenzoic acid hydrolyzing activity Priestia megaterium
V24F beta chain, no effect on the formation of cephalexin, 11% of nitrophenylacetylaminobenzoic acid hydrolyzing activity Priestia megaterium
Y144R alpha chain, reduced formation of cephalexin, 72% of nitrophenylacetylaminobenzoic acid hydrolyzing activity Priestia megaterium
Y144R/V24F Y144R on alpha chain, V24F on beta chain, reduced formation of cephalexin, 4% of nitrophenylacetylaminobenzoic acid hydrolyzing activity Priestia megaterium

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Priestia megaterium
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
penicillin G + H2O Priestia megaterium
-
6-aminopenicillanic acid + phenylacetic acid
-
?

Organism

Organism UniProt Comment Textmining
Priestia megaterium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native and mutant proteins Priestia megaterium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.01
-
nitrophenylacetylaminobenzoic acid as substrate, purified F145A mutant enzyme, 37°C Priestia megaterium
0.04
-
nitrophenylacetylaminobenzoic acid as substrate, purified F145L mutant enzyme, 37°C Priestia megaterium
0.85
-
7-amino-desacetoxycephalosporanic acid + D-phenylglycine amide as substrates, purified Y144R mutant enzyme, 30°C, pH 7.0 Priestia megaterium
0.904
-
7-amino-desacetoxycephalosporanic acid + D-phenylglycine amide as substrates, purified Y144R/V24F mutant enzyme, 30°C, pH 7.0 Priestia megaterium
1.22
-
nitrophenylacetylaminobenzoic acid as substrate, purified Y144R/V24F mutant enzyme, 37°C Priestia megaterium
1.48
-
7-amino-desacetoxycephalosporanic acid + D-phenylglycine amide as substrates, purified V24F mutant enzyme, 30°C, pH 7.0 Priestia megaterium
1.49
-
7-amino-desacetoxycephalosporanic acid + D-phenylglycine amide as substrates, purified native enzyme, 30°C, pH 7.0 Priestia megaterium
1.94
-
7-amino-desacetoxycephalosporanic acid + D-phenylglycine amide as substrates, purified F145Y mutant enzyme, 30°C, pH 7.0 Priestia megaterium
3.35
-
nitrophenylacetylaminobenzoic acid as substrate, purified V24F mutant enzyme, 37°C Priestia megaterium
9.73
-
nitrophenylacetylaminobenzoic acid as substrate, purified F145Y mutant enzyme, 37°C Priestia megaterium
22.1
-
nitrophenylacetylaminobenzoic acid as substrate, purified Y144R mutant enzyme, 37°C Priestia megaterium
30.6
-
nitrophenylacetylaminobenzoic acid as substrate, purified native enzyme, 37°C Priestia megaterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-nitro-3-(phenylacetamido)benzoic acid + H2O
-
Priestia megaterium phenylacetic acid + 5-amino-2-nitrobenzoic acid
-
?
7-amino-desacetoxycephalosporanic acid + D-phenylglycine amide high reactant concentrations Priestia megaterium cephalexin
-
r
cephalosporin G + H2O
-
Priestia megaterium 7-amino-desacetoxycephalosporanic acid + phenylacetic acid
-
?
penicillin G + H2O
-
Priestia megaterium 6-aminopenicillanic acid + phenylacetic acid
-
?

Subunits

Subunits Comment Organism
? alpha and beta chain Priestia megaterium

Synonyms

Synonyms Comment Organism
BmPGA
-
Priestia megaterium
penicillin G acylase
-
Priestia megaterium