Application | Comment | Organism |
---|---|---|
synthesis | purification of His-tagged enzyme secreted into periplasmic space by immobilized metal-ion affnity chromatography. Maximum specific activity of enzyme is 38.5 U/mg, yield is 70% | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purification of His-tagged enzyme secreted into periplasmic psace by immobilized metal-ion affnity chromatography. Maximum specific activity of enzyme is 38.5 U/mg, yield is 70% | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
38.5 | - |
40°C | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
penicillin G + H2O | - |
Escherichia coli | 6-aminopenicillanic acid + phenylacetic acid | - |
? |